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Titolo:
Evidence of macrophage foam cell formation by very low-density lipoproteinreceptor - Interferon-gamma inhibition of very low-density lipoprotein receptor expression and foam cell formation in macrophages
Autore:
Kosaka, S; Takahashi, S; Masamura, K; Kanehara, H; Sakai, J; Tohda, G; Okada, E; Oida, K; Iwasaki, T; Hattori, H; Kodama, T; Yamamoto, T; Miyamori, I;
Indirizzi:
Fukui Med Univ, Fac Med, Dept Internal Med 3, Matsuoka, Fukui, Japan FukuiMed Univ Matsuoka Fukui Japan ternal Med 3, Matsuoka, Fukui, Japan Tohoku Univ, Sch Med, Dept Internal Med 2, Sendai, Miyagi 980, Japan Tohoku Univ Sendai Miyagi Japan 980 rnal Med 2, Sendai, Miyagi 980, Japan BML Inc, Ctr Res & Dev, Res Dept, Kawagoe, Saitama, Japan BML Inc KawagoeSaitama Japan s & Dev, Res Dept, Kawagoe, Saitama, Japan Univ Tokyo, Adv Sci & Technol Res Ctr, Dept Mol Biol & Med, Tokyo, Japan Univ Tokyo Tokyo Japan chnol Res Ctr, Dept Mol Biol & Med, Tokyo, Japan Tohoku Univ, Ctr Gene Res, Sendai, Miyagi 980, Japan Tohoku Univ Sendai Miyagi Japan 980 r Gene Res, Sendai, Miyagi 980, Japan
Titolo Testata:
CIRCULATION
fascicolo: 8, volume: 103, anno: 2001,
pagine: 1142 - 1147
SICI:
0009-7322(20010227)103:8<1142:EOMFCF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; RABBIT ATHEROSCLEROTIC LESIONS; SCAVENGER RECEPTOR; APOLIPOPROTEIN-E; MESSENGER-RNA; LDL-RECEPTOR; CHOLESTEROL DEPOSITION; VLDL RECEPTOR; PROTEIN; DIFFERENTIATION;
Keywords:
lipoproteins; interferon-gamma; cells; receptors; proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, S Fukui Med Univ, Fac Med, Dept Internal Med 3, Matsuoka, Fukui, Japan Fukui Med Univ Matsuoka Fukui Japan Matsuoka, Fukui, Japan
Citazione:
S. Kosaka et al., "Evidence of macrophage foam cell formation by very low-density lipoproteinreceptor - Interferon-gamma inhibition of very low-density lipoprotein receptor expression and foam cell formation in macrophages", CIRCULATION, 103(8), 2001, pp. 1142-1147

Abstract

Background-Expression of the VLDL receptor, primarily in macrophages, has been confirmed in human and rabbit atherosclerotic lesions. The high binding affinity of the VLDL receptor for remnant particles implicates the VLDL receptor pathway in the foam cell formation mechanism in macrophages, This study investigates the effect of interferon (IFN)-gamma on VLDL receptor expression in phorbol-12-myristate-13-acetate (PMA)-treated THP-1, HL-60 macrophages, and human monocyte-derived macrophages,Methods and Results-THP-1 cells were induced to differentiate into macrophages by PMA treatment. IFN-gamma was added to the medium, and expression ofthe VLDL receptor was determined, I-125-beta -VLDL degradation study and oil red O staining were examined. In THP-1 macrophages, VLDL receptor protein expression decreased at 2 days after PMA treatment but increased at 3 days and increased up to 5 days, Scavenger receptor proteins, which were not originally present, appeared at 3 days after PMA treatment. IFN-gamma inhibited VLDL receptor expression in a dose-and time-dependent manner in macrophages, However, no inhibitory effect was observed in monocytes, Moreover, IFN-gamma receptor mRNA increased during differentiation to macrophages, I-125-beta -VLDL degradation study and oil red O staining showed that IFN-gammasignificantly inhibited foam cell formation after the uptake of beta -VLDL. LDL receptor-related protein (LRP) and LDL receptor mRNAs were not expressed in macrophages, In PMA-treated HL-60 macrophages and human monocyte-derived macrophages, IFN-gamma also inhibited VLDL receptor expression and foam cell formation by beta -VLDL. Conclusions-VLDL receptor expression is upregulated during monocyte-macrophage differentiation. IFN-gamma inhibits VLDL receptor expression and foam cell formation only in macrophages, Remnant particles induct macrophage foam cell formation through the VLDL receptor pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:57:31