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Titolo:
Adipocyte-derived plasma protein, adiponectin, suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages
Autore:
Ouchi, N; Kihara, S; Arita, Y; Nishida, M; Matsuyama, A; Okamoto, Y; Ishigami, M; Kuriyama, H; Kishida, K; Nishizawa, H; Hotta, K; Muraguchi, M; Ohmoto, Y; Yamashita, S; Funahashi, T; Matsuzawa, Y;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Internal Med & Mol Sci, Osaka, Japan Osaka Univ Osaka Japan h Med, Dept Internal Med & Mol Sci, Osaka, Japan Otsuka Pharmaceut Co Ltd, Cellular Technol Inst, Tokushima 77101, Japan Otsuka Pharmaceut Co Ltd Tokushima Japan 77101 t, Tokushima 77101, Japan
Titolo Testata:
CIRCULATION
fascicolo: 8, volume: 103, anno: 2001,
pagine: 1057 - 1063
SICI:
0009-7322(20010227)103:8<1057:APPASL>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY LIPOPROTEINS; NECROSIS-FACTOR-ALPHA; ATHEROSCLEROTIC LESIONS; GENE-EXPRESSION; OXIDIZED LDL; VISCERAL FAT; ADIPOSE; OBESITY; DIFFERENTIATION; DISEASE;
Keywords:
atherosclerosis; macrophages; receptors; adiponectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Ouchi, N Osaka Univ, Grad Sch Med, Dept Internal Med & Mol Sci, Osaka, Japan Osaka Univ Osaka Japan pt Internal Med & Mol Sci, Osaka, Japan
Citazione:
N. Ouchi et al., "Adipocyte-derived plasma protein, adiponectin, suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages", CIRCULATION, 103(8), 2001, pp. 1057-1063

Abstract

Background-Excessive lipid accumulation in macrophages plays an important role in the development of atherosclerosis. Recently, we discovered an adipocyte-specific plasma protein, adiponectin, that is decreased in patients with coronary artery disease. We previously demonstrated that adiponectin acts as a modulator for proinflammatory stimuli and inhibits monocyte adhesion to endothelial cells. The present study investigated the effects of adiponectin on lipid accumulation in human monocyte-derived macrophages. Methods and Results-Human monocytes were differentiated into macrophages by incubation in human type AB serum for 7 days, and the effects of adiponectin were investigated at different rime intervals. Treatment with physiological concentrations of adiponectin reduced intracellular cholesteryl ester content, as determined using the enzymatic. fluorometric method. The adiponectin-treated macrophages contained fewer lipid droplets stained by oil redO. Adiponectin suppressed the expression of the class A macrophage scavenger receptor (MSR) at both mRNA and protein levels by Northern acid immunoblot analyses, respectively, without affecting the expression of CD36, which was quantified by flow cytometry. Adiponectin reduced the class A MSR promoter activity, as measured by luciferase reporter assay. Adiponectin treatment dose-dependently decreased class A MSR ligand binding and uptake activities. The mRNA level of lipoprotein lipase as a marker of macrophage differentiation was decreased by adiponectin treatment, but that of apolipoproteinE was not altered. Adiponectin was detected around macrophages in the human injured aorta by immunohistochemistry. Conclusions-The adipocyte-derived plasma protein adiponectin suppressed macrophage-to-foam cell transformation, suggesting that adiponectin may act as a modulator for macrophage-to-foam cell transformation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 02:05:36