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Titolo:
Technetium-99m-tetrofosmin would be a substrate for multidrug resistance-associated protein (MRP): Comparison between a leukemia cell line with high MRP gene expression and its parental cell line
Autore:
Li, XF; Kinuya, S; Yokoyama, K; Konishi, S; Ma, YY; Watanabe, N; Shuke, N; Bunko, H; Michigishi, T; Tonami, N;
Indirizzi:
Kanazawa Univ, Sch Med, Dept Nucl Med, Kanazawa, Ishikawa 9208640, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9208640 wa, Ishikawa 9208640, Japan Toyama Med & Pharmaceut Univ, Toyama, Japan Toyama Med & Pharmaceut Univ Toyama Japan harmaceut Univ, Toyama, Japan Asahikawa Med Coll, Dept Radiol, Asahikawa, Hokkaido 078, Japan Asahikawa Med Coll Asahikawa Hokkaido Japan 078 kawa, Hokkaido 078, Japan Kanazawa Univ Hosp, Kanazawa, Ishikawa, Japan Kanazawa Univ Hosp KanazawaIshikawa Japan sp, Kanazawa, Ishikawa, Japan
Titolo Testata:
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
fascicolo: 1, volume: 16, anno: 2001,
pagine: 17 - 23
SICI:
1084-9785(200102)16:1<17:TWBASF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-TUMOR CELLS; P-GLYCOPROTEIN; IN-VITRO; CANCER; TC-99M-TETROFOSMIN; TC-99M-SESTAMIBI; TRANSPORTER; MECHANISM; MDR1;
Keywords:
multidrug resistance; multidrug resistance-associated protein; technetium-99m-tetrofosmin; verapamil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kinuya, S Kanazawa Univ, Sch Med, Dept Nucl Med, 13-1 Takaramachi, Kanazawa, Ishikawa 9208640, Japan Kanazawa Univ 13-1 Takaramachi Kanazawa IshikawaJapan 9208640 n
Citazione:
X.F. Li et al., "Technetium-99m-tetrofosmin would be a substrate for multidrug resistance-associated protein (MRP): Comparison between a leukemia cell line with high MRP gene expression and its parental cell line", CANC BIO R, 16(1), 2001, pp. 17-23

Abstract

The kinetics of cellular accumulation and retention of technetium-99m-tetrofosmin (Tc-99m-TF) were investigated in wild type HL60/WT cell line and inits doxorubicin-resistant HL60/DOX cell line with multidrug resistance-associated protein (MRP), but without P-gp overexpression, to determine whether Tc-99m-TF is a substrate for MRP. Methods: The accumulation and washout of Tc-99m-TF were observed in both cell lines at 37 degreesC. The effect of verapamil on the kinetics was also assessed. Results: Tc-99m-TF net accumulation was significantly lower in HL60/DOX (1.35 +/- 0.23%) than in HL60/WT (12.79 +/- 0.47%) at 60 min (P < 0.001). Three minutes after exchanging theincubation solution to the tracer-free medium only 18.20 +/- 0.34% of Tc-99m-TF remained in HL60/DOX, whereas 84.74 +/- 0.65% did in HL60/WT (P < 0.001). In the presence of 10 muM verapamil, Tc-99m-TF net accumulation in HL60/DOX was 302% of the control and the washout was significantly delayed. Conclusion: Tc-99m-TF would be a substrate for MRP and Tc-99m-TF may be used as a functional imaging agent of MRP in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 13:21:13