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Titolo:
Effect of prevention and potentiation of diisopropyl phosphorofluoridate (DFP)-induced delayed neurotoxicity on the mRNA expression of neurofilament subunits in hen central nervous system
Autore:
Xie, KQ; Gupta, RP; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27708 USA Duke Univ Durham NC USA 27708 Pharmacol & Canc Biol, Durham, NC 27708 USA Univ Sci & Technol China, Sch Life Sci, Neurotoxicol Lab, Hefei 230027, Anhui, Peoples R China Univ Sci & Technol China Hefei Anhui Peoples R China 230027 oples R China
Titolo Testata:
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
fascicolo: 2, volume: 79, anno: 2001,
pagine: 207 - 217
SICI:
0829-8211(200104)79:2<207:EOPAPO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; GENE-EXPRESSION; SPINAL-CORD; AXONAL NEUROFILAMENTS; NF-L; PATHOGENESIS; PROTEINS; CHICKEN; ATROPHY; NEURONS;
Keywords:
diisopropyl phosphorofluoridate; phenylmethylsulfonyl fluoride; hen; spinal cord; neurofilament mRNAs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27708USA Duke Univ POB 3813 Durham NC USA 27708 Durham, NC 27708USA
Citazione:
K.Q. Xie et al., "Effect of prevention and potentiation of diisopropyl phosphorofluoridate (DFP)-induced delayed neurotoxicity on the mRNA expression of neurofilament subunits in hen central nervous system", BIOC CELL B, 79(2), 2001, pp. 207-217

Abstract

Diisopropyl phosphorofluoridate (DFP) is an organophosphorus ester, which produces mild ataxia in 7-14 days and severe ataxia or paralysis in about 20 days (OPIDN) in hens. Previous studies in this laboratory have shown enhanced temporal expression of neurofilament (NF) subunit mRNAs in the spinal cord (SC) of DFP-treated hens. The main objective of this investigation wasto study the effect of DFP administration on NF subunit mRNAs expression, when OPIDN is protected or potentiated by pre-treatment or post-treatment, respectively, with phenylmethylsulfonyl fluoride (PMSF). The hens were sacrificed 1, 5, 10, and 20 days after the last treatment. In contrast with enhanced mRNA expression of NF subunits reported in OPIDN, there was no alteration in the expression of NF subunits in the SC of PMSF-protected hens thatdid not develop OPIDN. PMSF post-treatment of DFP-treated hens, which enhanced delayed neurotoxicity produced by a low dose of DFP, exhibited decrease in the mRNA expression of NF subunits in SC at all time periods (1-20 days) of observation. The expression of NF subunits was also studied in the degeneration-resistant tissue cerebrum of treated hens. The results from protected hens suggested that temporal enhanced expression of NF subunit mRNAs in DFP-treated hens might be contributing to the development of OPIDN in hens. By contrast, PMSF post-treatment seemed to potentiate OPIDN by a mechanism different from that followed by DFP alone to produce OPIDN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:14:33