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Titolo:
Lipid microspheres incorporated by U937 cells via their specific receptors
Autore:
Suzuki, K; Hidaka, T; Shinohara, T; Kataharada, K; Okada, M; Takada, K; Ohsuzu, F;
Indirizzi:
Natl Def Med Coll, Tokorozawa, Saitama 3598513, Japan Natl Def Med Coll Tokorozawa Saitama Japan 3598513 Saitama 3598513, Japan
Titolo Testata:
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
fascicolo: 2, volume: 79, anno: 2001,
pagine: 177 - 182
SICI:
0829-8211(200104)79:2<177:LMIBUC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; TRIGLYCERIDE-RICH LIPOPROTEINS; HUMAN ENDOTHELIAL-CELLS; ALPHA-2-MACROGLOBULIN RECEPTOR; MACROPHAGE RECEPTOR; APOLIPOPROTEIN-E; DRUG CARRIERS; LDL RECEPTOR; BINDING; PROTEIN;
Keywords:
lipid emulsion; drug delivery system; monocyte; free fatty acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, K Natl Def Med Coll, Namiki 3-2, Tokorozawa, Saitama 3598513, Japan Natl Def Med Coll Namiki 3-2 Tokorozawa Saitama Japan 3598513 an
Citazione:
K. Suzuki et al., "Lipid microspheres incorporated by U937 cells via their specific receptors", BIOC CELL B, 79(2), 2001, pp. 177-182

Abstract

Lipid microspheres (LM), currently in clinical use as drug carriers, mainly consist of soybean oil as a core and lecithin as a surfactant. The purpose of our study wass to determine whether or not LM incorporation is receptor-mediated. U937 cells resuspended in a serum-free medium abundantly took up unmodulated LM. A binding study showed that U937 cells had a single binding site for LM (410 sites/cell at 24 degreesC; 100 sites/cell at 4 degreesC). Inhibition assays revealed that lecithin liposome, lysophosphatidylcholines, activated alpha (2)-macroglobulin, and HDL did not affect the binding of LM to U937 cells. VLDL strongly, and LDL and AcLDL moderately, inhibitedthe binding of LM to U937 cells. Ligand blotting analysis revealed that unmodulated LM in an apoprotein-free buffer directly bound to a 40 kDa protein in the cell membrane fraction. These results suggest that LM that is not modulated by any protein is incorporated by specific cells via receptor-mediated processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:23:04