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Titolo:
Identification of the S6 kinase activity stimulated in quiescent brine shrimp embryos upon entry to preemergence development as p70 ribosomal proteinS6 kinase: Isolation of Artemia franciscana p70(S6k) cDNA
Autore:
Santiago, J; Sturgill, TW;
Indirizzi:
Univ Virginia, Hlth Sci Ctr, Ctr Cell Signaling, Dept Pharmacol, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Dept Med, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA
Titolo Testata:
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
fascicolo: 2, volume: 79, anno: 2001,
pagine: 141 - 152
SICI:
0829-8211(200104)79:2<141:IOTSKA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; GENE; PHOSPHORYLATION; EXPRESSION; SALINA; SITE;
Keywords:
p70 ribosomal S6 kinase cDNA; brine shrimp; development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Sturgill, TW Univ Virginia, Hlth Sci Ctr, Ctr Cell Signaling, Dept Pharmacol, Box 577, Charlottesville, VA 22908 USA Univ Virginia Box 577 Charlottesville VA USA 22908 22908 USA
Citazione:
J. Santiago e T.W. Sturgill, "Identification of the S6 kinase activity stimulated in quiescent brine shrimp embryos upon entry to preemergence development as p70 ribosomal proteinS6 kinase: Isolation of Artemia franciscana p70(S6k) cDNA", BIOC CELL B, 79(2), 2001, pp. 141-152

Abstract

We previously demonstrated that a protein kinase responsible for phosphorylating 40S ribosomal subunits is activated in quiescent Artemia franciscanaembryos within 15 min of restoration of normal tonicity and incubation at 30 degreesC. Here, we identify the activated S6 kinase as A. franciscana p70 ribosomal S6 kinase (p70(S6k)) subsequent to the isolation of an Artemia p70(S6k) cDNA. The protein conceptually translated from cDNA has 70% similarity and 64% identity to both Drosophila melanogaster and human p70(S6k). Southern blot analysis is consistent with presence of a single p70(S6k) gene. Two transcripts of 5.4 and 2.7 kb were found. Abundance of both mRNAs increased dramatically around 4 h of preemergence development, and exhibited different steady-state level variation thereafter. Stimulated S6 kinase activity, partially purified by Superose 6 chromatography, correlated best withthe slowest migrating, similar to 65 kDa, form detected by Western analysis using a specific polyclonal antibody made to a peptide from the predictedp70(S6k) NH2-terminus. Furthermore, the A. franciscana p70(S6k) was immunoprecipitated with the same antibody, showing in parallel an S6 kinase activity similar to peak profiles. We conclude that the stimulated S6 kinase activity is that of an ortholog of human p70(S6k) that may be involved in the regulation of protein synthesis during preemergence development in A. franciscana species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 01:05:09