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Titolo:
Inhibition of preadipocyte differentiation by myostatin treatment in 3T3-L1 cultures
Autore:
Kim, HS; Liang, L; Dean, RG; Hausman, DB; Hartzell, DL; Baile, CA;
Indirizzi:
Univ Georgia, Dept Anim & Dairy Sci, Athens, GA 30602 USA Univ Georgia Athens GA USA 30602 t Anim & Dairy Sci, Athens, GA 30602 USA Univ Georgia, Dept Foods & Nutr, Athens, GA 30602 USA Univ Georgia AthensGA USA 30602 Dept Foods & Nutr, Athens, GA 30602 USA MetaMorphix Inc, Baltimore, MD 21227 USA MetaMorphix Inc Baltimore MD USA21227 rphix Inc, Baltimore, MD 21227 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 281, anno: 2001,
pagine: 902 - 906
SICI:
0006-291X(20010309)281:4<902:IOPDBM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; GROWTH-FACTOR-BETA; BINDING PROTEIN-ALPHA; OB GENE-EXPRESSION; TGF-BETA; ADIPOCYTE DIFFERENTIATION; IN-VITRO; LEPTIN; ADIPOGENESIS; TRANSCRIPTION;
Keywords:
myostatin; CCAAT/enhancer-binding protein alpha (C/EBP alpha); peroxisome proliferator-activated receptor gamma (PPAR gamma); 3T3-L1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Baile, CA Univ Georgia, Dept Anim & Dairy Sci, 444 Anim Sci Complex, Athens, GA 30602 USA Univ Georgia 444 Anim Sci Complex Athens GA USA 30602 30602 USA
Citazione:
H.S. Kim et al., "Inhibition of preadipocyte differentiation by myostatin treatment in 3T3-L1 cultures", BIOC BIOP R, 281(4), 2001, pp. 902-906

Abstract

Myostatin, a new TGF-beta family member, is known as a muscle growth inhibitor, but its role in adipocyte development has not been studied. To test the role of Myostatin in 3T3-L1 preadipocyte differentiation, we treated cultured 3T3-L1 preadipocytes with Myostatin dissolved in 0.1% trifluoroaceticacid (TFA) during differentiation after they had become confluent. Myostatin treatment significantly decreased glycerol-3-phosphate dehydrogenase (GPDH) activity and oil Red-O staining compared to controls that did not receive Myostatin. Western blot analysis showed that the expression levels of CCAAT-enhancer binding protein a! (C/EBP alpha) and peroxisome proliferator-activated receptor gamma (PPAR gamma) were significantly decreased by Myostatin treatment (P < 0.05). However, the expression of C/EBP <beta> was not significantly changed by the treatment (P > 0.05). From RT-PCR result, the relative level of leptin mRNA in Myostatin-treated cells was not significantly different (P > 0.1) from the level in cells without Myostatin treatment. Our data show that Myostatin, a secreted protein from muscle, inhibits preadipocyte differentiation in 3T3-L1 cells, which is mediated, in part, by altered regulation of C/EBP a! and PPAR gamma. (C) 2001 Academic Press.

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Documento generato il 01/04/20 alle ore 21:02:34