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Titolo:
Synergistic activation of RSK correlates with c-fos induction in MO7e cells stimulated with GM-CSF plus steel factor
Autore:
Lee, Y; Broxmeyer, HE;
Indirizzi:
Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ol Ctr, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Immunol Microbiol, Dept Med, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 pt Med, Indianapolis, IN 46202 USA Walther Canc Inst, Indianapolis, IN 46208 USA Walther Canc Inst Indianapolis IN USA 46208 t, Indianapolis, IN 46208 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 281, anno: 2001,
pagine: 897 - 901
SICI:
0006-291X(20010309)281:4<897:SAORCW>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; PROTEIN; KINASE; INVOLVEMENT; GENE; PHOSPHORYLATION; TRANSCRIPTION; PROLIFERATION; SURVIVAL; LINE;
Keywords:
GM-CSF; steel factor; synergy; RSK; c-fos; MAP kinase; PI3 kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Broxmeyer, HE Indiana Univ, Sch Med, Walther Oncol Ctr, Bldg R4,Room 302,1044 W Walnut St, Indianapolis, IN 46202 USA Indiana Univ Bldg R4,Room 302,1044 W Walnut St Indianapolis IN USA 46202
Citazione:
Y. Lee e H.E. Broxmeyer, "Synergistic activation of RSK correlates with c-fos induction in MO7e cells stimulated with GM-CSF plus steel factor", BIOC BIOP R, 281(4), 2001, pp. 897-901

Abstract

Steel factor (SLF) plus GM-CSF induces proliferative synergy in factor-dependent cell line MO7e and hematopoietic progenitor cells. We previously reported ERK1/2 involvement in this synergy, but its downstream signaling molecules are not defined. Here, we investigated activation of the 90-kDa ribosomal S6 kinase (RSK) proteins by measuring the phosphorylation status and in vitro kinase activity in MO7e cells. Both GM-CSF and SLF induced activation of RSK, and the combined stimulation with these two cytokines induced synergistic and persistent activation of RSK, RSK activity was reduced by PI3kinase inhibitor LY294002 or MEK1 inhibitor PD98059, suggesting that the ERK as well as the PI3 kinase pathways are involved in regulation of RSK activity. Sensitivities of RSK activity to inhibitory drugs correlated well with those of c-fos gene induction. Taken together, synergistic activation ofRSK may contribute, at least in part, to the synergistic induction of c-fos after combined stimulation with GM-CSF plus SLF. (C) 2001 Academic Press.

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Documento generato il 09/04/20 alle ore 20:10:40