Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mitochondrial protein p32 can accumulate in the nucleus
Autore:
Brokstad, KA; Kalland, KH; Russell, WC; Matthews, DA;
Indirizzi:
St James Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England St James Univ Hosp Leeds W Yorkshire England LS9 7TF W Yorkshire, England Univ Bergen, Broegelmann Res Lab, N-5021 Bergen, Norway Univ Bergen Bergen Norway N-5021 egelmann Res Lab, N-5021 Bergen, Norway Univ Bergen, Gade Inst, Ctr Virol, Dept Microbiol & Immunol, N-5020 Bergen, Norway Univ Bergen Bergen Norway N-5020 robiol & Immunol, N-5020 Bergen, Norway Univ St Andrews, St Andrews KY16 9ST, Fife, Scotland Univ St Andrews St Andrews Fife Scotland KY16 9ST Y16 9ST, Fife, Scotland
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 5, volume: 281, anno: 2001,
pagine: 1161 - 1169
SICI:
0006-291X(20010316)281:5<1161:MPPCAI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; RNA-BINDING-PROTEINS; IN-VITRO INTERACTION; SPLICING FACTOR; ACTIVATION DOMAIN; CELLULAR PROTEIN; REV PROTEIN; TAT TRANSACTIVATOR; MESSENGER-RNA; P-32 PROTEIN;
Keywords:
p32; splicing associated protein; mitochondrial protein; actinomycin D (act. D); leptomycin B (LMB);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Matthews, DA St James Univ Hosp, Mol Med Unit, Clin Sci Bldg, Leeds LS9 7TF, W Yorkshire, England St James Univ Hosp Clin Sci Bldg Leeds W Yorkshire England LS9 7TF
Citazione:
K.A. Brokstad et al., "Mitochondrial protein p32 can accumulate in the nucleus", BIOC BIOP R, 281(5), 2001, pp. 1161-1169

Abstract

Human p32 was first isolated associated with the splicing factor ASF/SF-2,The p32 protein is translated as pre-protein from which a mitochondrial import signal is cleaved off to create the mature p32. The majority of p32 isconsequently found in the mitochondria. In this study we investigated extramitochondrial p32. An increased nuclear localisation of endogenous p32 wasdemonstrated as a response to leptomycin B or actinomycin D treatment of cells. Mature p32 gene and deletion mutants were cloned into enhanced green fluorescence protein reporter plasmids. On transfection, EGFP-p32 protein was mainly localised to the cytoplasm and to a lesser extent to the nucleus of transfected COS cells. Upon treatment with actinomycin D or leptomycin B, the EGFP-p32 protein accumulated in the nucleus. Deletion analysis indicated which regions of EGFP-p32 are involved in nuclear export and nuclear import. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:34:06