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Titolo:
Diffusion limitation causes decreased enantioselectivity of esterificationof 2-butanol by immobilized Candida antarctica lipase B
Autore:
Litjens, MJJ; Le, KQ; Straathof, AJJ; Jongejan, JA; Heijnen, JJ;
Indirizzi:
Delft Univ Technol, Kluyver Lab Biotechnol, NL-2628 BC Delft, Netherlands Delft Univ Technol Delft Netherlands NL-2628 BC 28 BC Delft, Netherlands
Titolo Testata:
BIOCATALYSIS AND BIOTRANSFORMATION
fascicolo: 1, volume: 19, anno: 2001,
pagine: 1 - 19
SICI:
1024-2422(2001)19:1<1:DLCDEO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYMATIC KINETIC RESOLUTION; CATALYZED RESOLUTION; WATER ACTIVITY; ORGANIC MEDIA; REACTOR;
Keywords:
kinetic resolution; enantioselectivity; diffusion limitation; particle size; immobilized enzyme; Candida antarctica lipase B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Straathof, AJJ Delft Univ Technol, Kluyver Lab Biotechnol, Julianalaan 67,NL-2628 BC Delft, Netherlands Delft Univ Technol Julianalaan 67 Delft Netherlands NL-2628 BC
Citazione:
M.J.J. Litjens et al., "Diffusion limitation causes decreased enantioselectivity of esterificationof 2-butanol by immobilized Candida antarctica lipase B", BIOCATAL B, 19(1), 2001, pp. 1-19

Abstract

Diffusion limitation in immobilized enzyme particles may decrease the performance of kinetic resolution processes. In this paper a standard reaction plus diffusion model approach, based on homogeneous enzyme distribution, isused to verify experimentally the influence of the particle diameter on the observed enantioselectivity. This is done for a commercial preparation ofimmobilized Candida antarctica lipase B in the kinetic resolution of racemic 2-butanol by esterification with vinyl butyrate in heptane. For immobilized enzyme particles with particle diameters ranging from 355 - 710 mum theapparent enantiomeric ratio is constant at E-app = 2.5, while for ground particles with a particle diameter of 1 mum the intrinsic enantiomeric ratiois E=4.4. This clear example of the influence of diffusion limitation on the observed enantioselectivity can not be described quantitatively in a straightforward way, because the enzyme seems to be present as a layer in the carrier particles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:14:11