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Titolo:
Vascular cell adhesion molecule-1 augments adenovirus-mediated gene transfer
Autore:
Chu, Y; Heistad, DD; Cybulsky, MI; Davidson, BL;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Neurol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Dept Neurol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Pharmacol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Dept Pharmacol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Ctr Cardiovasc, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Ctr Cardiovasc, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Ctr Aging, Iowa City, IA USA Univ Iowa Iowa City IA USA Iowa, Coll Med, Ctr Aging, Iowa City, IA USA Univ Iowa, Coll Med, Program Gene Therapy, Iowa City, IA USA Univ Iowa Iowa City IA USA Med, Program Gene Therapy, Iowa City, IA USA Vet Adm Med Ctr, Iowa City, IA USA Vet Adm Med Ctr Iowa City IA USAVet Adm Med Ctr, Iowa City, IA USA Univ Toronto, Toronto Hosp, Dept Lab Med & Pathobiol, Toronto, ON, Canada Univ Toronto Toronto ON Canada Lab Med & Pathobiol, Toronto, ON, Canada
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 2, volume: 21, anno: 2001,
pagine: 238 - 242
SICI:
1079-5642(200102)21:2<238:VCAMAA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL-CELLS; TYPE-5 FIBER; SUBGROUP-C; IN-VITRO; RECEPTOR; EXPRESSION; COXSACKIEVIRUS; ATTACHMENT; BINDING; PROTEIN;
Keywords:
vascular cell adhesion molecule-1; coxsackie and adenovirus receptor; adenovirus-mediated gene transfer; atherosclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Davidson, BL Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Iowa City, IA 52242 USA
Citazione:
Y. Chu et al., "Vascular cell adhesion molecule-1 augments adenovirus-mediated gene transfer", ART THROM V, 21(2), 2001, pp. 238-242

Abstract

We have reported that adenovirus-mediated gene transfer is augmented in the endothelium of atherosclerotic blood vessels. We observed that vascular cell adhesion molecule-1 (VCAM-1) shares some homology with the coxsackievirus and adenovirus receptor. Because VCAM-1 is upregulated on atherosclerotic endothelial cells, we hypothesized that VCAM-1 may act as an auxiliary receptor to augment adenovirus-mediated gene transfer. To test this hypothesis, stable NIH 3T3 cell lines that constitutively express VCAM-1 on the cellsurface were generated. Recombinant adenovirus 5 (Ad5), which contains thereporter beta -galactosidase gene, was used to compare Ad5 infection in VCAM-1(+) and parental NIH 3T3 cells. Total beta -galactosidase activity and the number of transgene-positive cells were 6- to 10-fold and 5-fold higher, respectively, in VCAM-1(+) than in VCAM-1(-) cells. Ad5 binding to VCAM-1(+) cells was increased by 3-fold over VCAM-1(-) cells. Soluble VCAM-1 protein, present during infection or viral binding, reduced P-galactosidase activity in VCAM-1(+) cells in a dose-dependent manner. Taken together, we conclude that VCAM-1 can mediate adenovirus binding and infection. This may explain, in part, the previous finding that adenovirus-mediated gene transferis augmented in atherosclerotic arteries.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:21:19