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Titolo:
Role of Sp1 in the induction of p27 gene expression in vascular smooth muscle cells in vitro and after balloon angioplasty
Autore:
Andres, V; Urena, J; Poch, E; Chen, DH; Goukassian, D;
Indirizzi:
Consejo Super Invest Cient, Inst Biomed, Unit Vasc Biol, Valencia 46010, Spain Consejo Super Invest Cient Valencia Spain 46010 l, Valencia 46010, Spain Tufts Univ, Sch Med, St Elizabeths Med Ctr, Dept Med Cardiol, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 tr, Dept Med Cardiol, Boston, MA 02111 USA
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 3, volume: 21, anno: 2001,
pagine: 342 - 347
SICI:
1079-5642(200103)21:3<342:ROSITI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT KINASE INHIBITOR; RAT CAROTID-ARTERY; CELLULAR PROLIFERATION; TRANSCRIPTION FACTOR; BREAST-CANCER; TIME-COURSE; CYCLIN-E; P27(KIP1); PROMOTER; GROWTH;
Keywords:
vascular smooth muscle cell; cell cycle; Sp1; p27; angioplasty;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Andres, V Consejo Super Invest Cient, Inst Biomed, Unit Vasc Biol, C JaimeRoig 11, Valencia 46010, Spain Consejo Super Invest Cient C Jaime Roig 11 Valencia Spain 46010
Citazione:
V. Andres et al., "Role of Sp1 in the induction of p27 gene expression in vascular smooth muscle cells in vitro and after balloon angioplasty", ART THROM V, 21(3), 2001, pp. 342-347

Abstract

The abnormal proliferation of vascular smooth muscle cells (VSMCs) plays an important role in atherosclerosis and restenosis. Although several studies have implicated the growth inhibitory protein p27(Kip1) (p27) in the control of myocyte growth and hypertrophy, Little is known about the molecular mechanisms that regulate p27 expression in the cardiovascular system. In the present study, we demonstrate the interaction of the transcription factorSp1 with 2 GC-rich sequences within the p27 promoter in cultured VSMCs. Importantly, point mutations that disrupted Sp1 binding markedly reduced p27 promoter activity, demonstrating that Sp1 is required for efficient p27 gene transcription in cultured VSMCs. Because p27 expression is upregulated after balloon angioplasty, we investigated Sp1 expression and activity in control and balloon-injured rat carotid arteries to assess the role of Sp1 as a physiological regulator of p27 expression. Although immunohistochemical analysis disclosed Sp1 protein expression in both control and balloon-injured arteries, a high level of Sp1 DNA-binding activity was found only in response to balloon angioplasty. Collectively, these results demonstrate that Sp1 is essential for maximum p27 promoter activity in VSMCs and suggest thatposttranslational induction of Sp1 DNA-binding activity contributes to theinduction of p27 expression and VSMC growth arrest at late time points after balloon angioplasty.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:05:00