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Titolo:
Expression of PTHrP and PTHR (PTH/PTHrP-r) mRNAs and polypeptides in bovine ovary and stimulation of bovine blastocyst development in vitro followingPTHrP treatment during oocyte maturation
Autore:
Watson, PH; Westhusin, ME; Watson, AJ;
Indirizzi:
Univ Western Ontario, Dept Obstet & Gynaecol, London, ON NGA 5C1, Canada Univ Western Ontario London ON Canada NGA 5C1 London, ON NGA 5C1, Canada Univ Western Ontario, Dept Physiol, London, ON NGA 5C1, Canada Univ Western Ontario London ON Canada NGA 5C1 London, ON NGA 5C1, Canada Univ Western Ontario, Dept Med, London, ON NGA 5C1, Canada Univ Western Ontario London ON Canada NGA 5C1 London, ON NGA 5C1, Canada St Josephs Hlth Ctr, Lawson Res Inst, London, ON, Canada St Josephs Hlth Ctr London ON Canada Lawson Res Inst, London, ON, Canada Texas A&M Univ, Coll Vet Med, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 rmacol, College Stn, TX 77843 USA
Titolo Testata:
ANATOMY AND EMBRYOLOGY
fascicolo: 3, volume: 203, anno: 2001,
pagine: 175 - 184
SICI:
0340-2061(200103)203:3<175:EOPAP(>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-RELATED PROTEIN; HUMAN PARATHYROID-HORMONE; RECEPTOR MESSENGER-RNA; PARIETAL ENDODERM DIFFERENTIATION; GROWTH-FACTOR-BETA; IN-VITRO; PEPTIDE RECEPTOR; INVITRO FERTILIZATION; NUCLEAR-LOCALIZATION; RIBONUCLEIC-ACIDS;
Keywords:
blastocyst; in vitro fertilization; growth factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Watson, AJ Univ Western Ontario, Dept Obstet & Gynaecol, London, ON NGA 5C1, Canada Univ Western Ontario London ON Canada NGA 5C1 NGA 5C1, Canada
Citazione:
P.H. Watson et al., "Expression of PTHrP and PTHR (PTH/PTHrP-r) mRNAs and polypeptides in bovine ovary and stimulation of bovine blastocyst development in vitro followingPTHrP treatment during oocyte maturation", ANAT EMBRYO, 203(3), 2001, pp. 175-184

Abstract

Parathyroid hormone related protein (PTHrP) and its receptor have well-established roles in the development and regulation of many tissues, includingbone and mammary gland. The objectives of this study were: (1) to characterize the distribution of mRNAs encoding parathyroid hormone (PTH)-related protein (PTHrP) and receptor (PTHR) in bovine ovary; (2) to characterize thedistribution of PTHrP and PTHR polypeptides in bovine ovary; (3) to examine the influences of PTHrP (1-141) treatment during bovine oocyte maturationin vitro on blastocyst development. mRNAs encoding PTHrP and PTHR were detected by in situ hybridization methods in oocytes, and granulosa cells in all follicles from primordial to large antral. PTHrP and PTHR polypeptides displayed distinct distribution patterns with PTHrP polypeptides primarily confined to oocytes from primordial to large antral follicles. PTHrP polypeptides were detectable but at a reduced level in ovarian stroma and in granulosa and thecal layers. PTHR polypeptides were detected in oocytes of all follicular stages but were predominantly found in ovarian stroma, granulosa and theca follicular layers. Supplementation of serum-free cSOFMaa oocyte maturation medium with PTHrP (1-141) resulted in a concentration-dependent increase in development to the blastocyst stage in vitro. The results suggest that granulosa cells may be a primary site of PTHrP production and release. Oocytes from all follicular stages stained strongly for PTHrP polypeptides and PTHrP enhanced development to the blastocyst stage in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 15:09:39