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Titolo:
SCFA increase intestinal Na absorption by induction of NHE3 in rat colon and human intestinal C2/bbe cells
Autore:
Musch, MW; Bookstein, C; Xie, Y; Sellin, JH; Chang, EB;
Indirizzi:
Univ Chicago, Martin Boyer Labs, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 artin Boyer Labs, Chicago, IL 60637 USA Univ Texas, Sch Med, Gastroenterol Sect, Houston, TX 77225 USA Univ TexasHouston TX USA 77225 Gastroenterol Sect, Houston, TX 77225 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 4, volume: 280, anno: 2001,
pagine: G687 - G693
SICI:
0193-1857(200104)280:4<G687:SIINAB>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN FATTY-ACIDS; EPITHELIAL-CELLS; ELECTROLYTE TRANSPORT; SODIUM-BUTYRATE; IN-VITRO; EXPRESSION; GENE; DIFFERENTIATION; INHIBITION; MODULATION;
Keywords:
short-chain fatty acids; butyrate; sodium transport; nutrition; gene induction; intestinal adaptation; dietary fiber; colonocyte; Na/H exchanger 3; Na/H exchanger 2; intestinal flora; bacteria; intestinal mucosa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, EB Univ Chicago, Martin Boyer Labs, MC6084,5841 S Maryland Ave, Chicago, IL 60637 USA Univ Chicago MC6084,5841 S Maryland Ave Chicago IL USA 60637 USA
Citazione:
M.W. Musch et al., "SCFA increase intestinal Na absorption by induction of NHE3 in rat colon and human intestinal C2/bbe cells", AM J P-GAST, 280(4), 2001, pp. G687-G693

Abstract

Short-chain fatty acids (SCFA), produced by colonic bacterial flora fermentation of dietary carbohydrates, promote colonic Na absorption through mechanisms not well understood. We hypothesized that SCFA promote increased expression of apical membrane Na/H exchange (NHE), serving as luminal physiological cues for regulating colonic Na absorptive capacity. Studies were performed in human colonic C2/bbe (C2) monolayers and in vivo. In C2 cells exposed to butyrate, acetate, proprionate, or the poorly metabolized SCFA isobutyrate, apical membrane NHE3 activity and protein expression increased in atime- and concentration-dependent manner, whereas no changes were observedfor NHE2. In contrast, no significant changes in brush-border hydrolase orvillin expression were noted. Analogous to the in vitro findings, rats fedthe soluble fiber pectin exhibited a time- dependent increase in colonic NHE3, but not NHE2, protein, mRNA, and brush-border activity. These changes were region-specific, as no changes were observed in the ileum. We concludethat luminal SCFA are important physiological cues for regulating colonic Na absorptive function, allowing the colon to adapt to chronic changes in dietary carbohydrate and Na loads.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:05:30