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Titolo:
Latex based, rapid and easy assay for human leptospirosis in a single testformat
Autore:
Smits, HL; Chee, HD; Eapen, CK; Kuriakose, M; Sugathan, S; Gasem, MH; Yersin, C; Sakasi, D; Lai-a-Fat, RFM; Hartskeerl, RA; Liesdek, B; Abdoel, TH; Goris, MGA; Gussenhoven, GC;
Indirizzi:
Royal Trop Inst, Dept Biomed Res, NL-1105 AZ Amsterdam, Netherlands Royal Trop Inst Amsterdam Netherlands NL-1105 AZ Amsterdam, Netherlands Acad Hosp, Dept Dermatol, Paramaribo, Surinam Acad Hosp Paramaribo Surinam d Hosp, Dept Dermatol, Paramaribo, Surinam Malankara Orthodox Syrian Church Med Mission Hosp, Kolenchery, India Malankara Orthodox Syrian Church Med Mission Hosp Kolenchery India ndia Diponegoro Univ, Dr Kariadi Hosp, Dept Med, Semarang, Indonesia DiponegoroUniv Semarang Indonesia Hosp, Dept Med, Semarang, Indonesia Victoria Hosp, Dept Med, Victoria, Seychelles Victoria Hosp Victoria Seychelles Hosp, Dept Med, Victoria, Seychelles Dept Hlth, Epidemiol Branch, Honolulu, HI USA Dept Hlth Honolulu HI USADept Hlth, Epidemiol Branch, Honolulu, HI USA Acad Hosp, Dept Internal Med, Paramaribo, Surinam Acad Hosp Paramaribo Surinam sp, Dept Internal Med, Paramaribo, Surinam
Titolo Testata:
TROPICAL MEDICINE & INTERNATIONAL HEALTH
fascicolo: 2, volume: 6, anno: 2001,
pagine: 114 - 118
SICI:
1360-2276(200102)6:2<114:LBRAEA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERNATIONAL MULTICENTER EVALUATION; INDIRECT HEMAGGLUTINATION ASSAY; IMMUNOGLOBULIN-M ANTIBODIES; LINKED-IMMUNOSORBENT-ASSAY; DIPSTICK ASSAY; LEPTO DIPSTICK; HUMAN-SERUM; DIAGNOSIS;
Keywords:
leptospirosis; latex agglutination assay; serodiagnosis; microscopic agglutination assay; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Smits, HL Royal Trop Inst, Dept Biomed Res, Meibergdreef 39, NL-1105 AZ Amsterdam, Netherlands Royal Trop Inst Meibergdreef 39 Amsterdam NetherlandsNL-1105 AZ
Citazione:
H.L. Smits et al., "Latex based, rapid and easy assay for human leptospirosis in a single testformat", TR MED I H, 6(2), 2001, pp. 114-118

Abstract

Leptospirosis is an often severe disease which requires prompt treatment. Laboratory testing is required to reach a valid diagnosis. An agglutinationassay for the detection of Leptospira-specific antibodies consisting of individually wrapped agglutination cards containing a stable, dried detectionreagent is evaluated. The assay is simply performed by suspending the dried reagent with a drop of serum. The result is obtained within 30 s. The sensitivity of the assay varied with the stage of the disease and was 72.3% for samples collected during the first 10 days of the illness and 88.2% for samples collected at a later stage. The specificity was 93.9% and 89.8%, respectively. These characteristics make the test ideal for use in areas wherethe disease is common and where laboratory support is not routinely available.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/06/20 alle ore 05:08:54