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Titolo:
Dextran sedimentation in a semi-closed system for the clinical banking of umbilical cord blood
Autore:
Tsang, KS; Li, K; Huang, DP; Wong, AP; Leung, Y; Lau, TT; Chang, AMZ; Li, CK; Fok, TF; Yuen, PMP;
Indirizzi:
Chinese Univ Hong Kong, Prince Wales Hosp, Lady Paos Children Canc Ctr, Dept Anat & Cellular Pathol,Div Bone Marrow Trans, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China Chinese Univ Hong Kong, Prince Wales Hosp, Dept Pediat, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China Chinese Univ Hong Kong, Prince Wales Hosp, Dept Obstet & Gynecol, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China
Titolo Testata:
TRANSFUSION
fascicolo: 3, volume: 41, anno: 2001,
pagine: 344 - 352
SICI:
0041-1132(200103)41:3<344:DSIASS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW RECONSTITUTION; STEM-CELLS; DENSITY-SEPARATION; ANAPHYLACTOID REACTIONS; PROGENITOR CELLS; CRYOPRESERVATION; TRANSPLANTATION; RECOVERY; DEPLETION; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Tsang, KS Chinese Univ Hong Kong, Prince Wales Hosp, Lady Paos Children Canc Ctr, Dept Anat & Cellular Pathol,Div Bone Marrow Trans, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples RChina ina
Citazione:
K.S. Tsang et al., "Dextran sedimentation in a semi-closed system for the clinical banking of umbilical cord blood", TRANSFUSION, 41(3), 2001, pp. 344-352

Abstract

BACKGROUND: The results of current processing procedures for reducing volume and recovering HPCs from umbilical cord blood (UCB) before cryopreservation vary. STUDY DESIGN AND METHODS: Dextran was added to bags containing UCB, followed by sedimentation for 30 minutes. The processed UCB was then frozen. RBCs, nucleated cells, MNCs, CD34+ cells, CFUs and long-term culture-initiatingcells (LTC-ICs), viability, and sterility were evaluated. Fractionations in ficoll-hypaque and hydroxyethyl starch (HES) were also run in parallel for comparison. RESULTS: The nucleated cell (NC) recovery and RBC depletion were 86.1 percent and 94.3 percent, respectively (n = 50). Sedimentation with dextran also enabled the recovery of 80.7 percent MNCs and 82.6 percent CD34+ cells (n= 30). Postsedimentation samples displayed no impairment of CFU growth (n = 42, 108.7% CFU-C, 104.6% CFU-GEMM, 107% CFU-GM, and 95.7% BFU-E). Long-term cultures on five paired samples before and after sedimentation generatedsimilar numbers of CFU-C each week (p = 0.88). Limiting dilution analysis of 12 paired pre/postsedimentation samples showed comparable median proportions of LTC-ICs (1/6494 vs. 1/5236; p = 0.18). The cell viability of 24 samples of thawed UCB after sedimentation was 90.3 percent (77.5-96%) and the recovery of CFU-C, CFU-GEMM, CFU-GM, and BFU-E of 11 postsedimentation samples was 93.4 percent, 84.9 percent, 92.3 percent, and 83.4 percent, respectively. NC recovery was significantly higher after treatment with dextran than with ficoll-hypaque (n = 30; 88.5% vs. 29.1%; p<0.005) and HES treatment(n = 21;88.5% vs. 76.4%; p=0.004). However, MNCs, CD34+ cells, CFUs, LTC-ICs, and RBCs were comparable. Two cycles of dextran Sedimentation recovered93.9 percent of NCs with cell viability of 98.6 percent (96.5-100%), whereas 11.7 percent of RBCs were retained (n = 20). The final yield volume was 33.5 (28-41) mL. CONCLUSION: In a semi-closed system, dextran sedimentation enabled volume reduction of UCB without significant quantitative and qualitative losses ofHPCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:33:04