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Titolo:
Rho-kinase is involved in the sustained phosphorylation of myosin and the irreversible platelet aggregation induced by PAR1 activating peptide
Autore:
Missy, K; Plantavid, M; Pacaud, P; Viala, C; Chap, H; Payrastre, B;
Indirizzi:
Hop Purpan, INSERM, U326, Inst Federat Rech 30, F-31059 Toulouse, France Hop Purpan Toulouse France F-31059 rat Rech 30, F-31059 Toulouse, France Fac Sci & Tech Nantes, INSERM, U533, Nantes, France Fac Sci & Tech NantesNantes France antes, INSERM, U533, Nantes, France
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 3, volume: 85, anno: 2001,
pagine: 514 - 520
SICI:
0340-6245(200103)85:3<514:RIIITS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRESS FIBER FORMATION; HUMAN BLOOD-PLATELETS; NA-H EXCHANGE; SHAPE CHANGE; ACTIN CYTOSKELETON; PROTEIN-KINASE; PHOSPHOINOSITIDE 3-KINASE; SIGNAL-TRANSDUCTION; FOCAL ADHESIONS; SMOOTH-MUSCLE;
Keywords:
Rho-kinase; irreversible platelet aggregation; myosin; TRAP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Payrastre, B Hop Purpan, INSERM, U326, Inst Federat Rech 30, F-31059 Toulouse, France Hop Purpan Toulouse France F-31059 F-31059 Toulouse, France
Citazione:
K. Missy et al., "Rho-kinase is involved in the sustained phosphorylation of myosin and the irreversible platelet aggregation induced by PAR1 activating peptide", THROMB HAEM, 85(3), 2001, pp. 514-520

Abstract

We have addressed the role of Rho-kinase in the different steps of thrombin receptor agonist peptide (TRAP)-induced platelet activation. Interestingly, under physiological conditions, incubation of platelets with increasing concentrations of the specific Rho-kinase inhibitor Y-27632 resulted in a dose-dependent reversion of the aggregation induced by 10 muM TRAP, without affecting serotonin secretion. Addition of Y-27632 after three minutes of TRAP stimulation, when the maximal aggregation was reached, resulted in a rapid disaggregation of platelets. Accordingly, the early peak of myosin light chain (MLC) phosphorylation induced by TRAP was not affected by Y-27632 but its sustained phosphorylation, observed during the irreversible phase ofaggregation, was dependent of Rho-kinase activity. The rapid decrease in MLC phosphorylation upon Y-27632 treatment correlated well with the specificdisappearance of myosin heavy chain from the cytoskeleton and preceded platelet disaggregation. Finally, we provide evidence that secreted ADP, knownto play a key role in TRAP-induced irreversible phase of aggregation, was involved in the sustained MLC phosphorylation through Rho-kinase and could be replaced by epinephrine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:58:04