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Titolo:
Performance characteristics of four free phenytoin immunoassays
Autore:
Roberts, WL; Annesley, TM; De, BK; Moulton, L; Juenke, JM; Moyer, TP;
Indirizzi:
ARUP Labs, Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USA ARUP Labs Salt Lake City UT USA 84108 athol, Salt Lake City, UT 84108 USA Univ Utah, Hlth Sci Ctr, Dept Pathol, Salt Lake City, UT USA Univ Utah Salt Lake City UT USA Ctr, Dept Pathol, Salt Lake City, UT USA Univ Michigan, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 iv Michigan, Ann Arbor, MI 48109 USA Univ Mississippi, Med Ctr, Jackson, MS 39216 USA Univ Mississippi JacksonMS USA 39216 ppi, Med Ctr, Jackson, MS 39216 USA Mayo Clin, Lab Med & Pathol, Rochester, MN USA Mayo Clin Rochester MN USA ayo Clin, Lab Med & Pathol, Rochester, MN USA
Titolo Testata:
THERAPEUTIC DRUG MONITORING
fascicolo: 2, volume: 23, anno: 2001,
pagine: 148 - 154
SICI:
0163-4356(200104)23:2<148:PCOFFP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; UREMIC PATIENTS; INTERFERENCE; METABOLITE; SERUM;
Keywords:
fluorescence polarization immunoassay; free phenytoin; immunoassay; metabolite interference; uremia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, WL ARUP Labs, Inst Clin & Expt Pathol, 500 Chipeta Way, Salt LakeCity, UT 84108 USA ARUP Labs 500 Chipeta Way Salt Lake City UT USA 84108 4108 USA
Citazione:
W.L. Roberts et al., "Performance characteristics of four free phenytoin immunoassays", THER DRUG M, 23(2), 2001, pp. 148-154

Abstract

The measurement of the unbound or free phenytoin concentration is indicated in several situations, including uremia. In patients with uremia, metabolites of phenytoin and other substances accumulate and can displace phenytoin from its protein binding sites, with a consequent increase in the free fraction of drug. Some of the phenytoin metabolites that accumulate in uremiacan cross-react with phenytoin immunoassays. In this study the authors evaluated four free phenytoin immunoassays compared with a high-performance liquid chromatography (HPLC) method: the Roche COBAS Integra, the Syva EMIT 2000, the Opus INNOFLUOR, and the Abbott TDx. All four methods demonstrated good precision, with interday coefficients of variation of less than or equal to 5% and comparable recoveries using quality control material. Two of the methods, the EMIT 2000 and COBAS Integra, showed excellent agreement with the HPLC method using samples from patients both with normal renal function and with renal insufficiency. The other two methods, the INNOFLUOR and TDx, showed average positive biases for the therapeutic range of 3-7% and 21-22%, respectively, compared with the HPLC method for samples from patientswith normal renal function, and average positive biases of 24-32% and 75-81%, respectively, with samples from patients with uremia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 18:18:33