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Titolo:
Negative autoregulation of the Arabidopsis homeobox gene ATHB-2
Autore:
Ohgishi, M; Oka, A; Morelli, G; Ruberti, I; Aoyama, T;
Indirizzi:
Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan Kyoto Univ Uji KyotoJapan 6110011 st Chem Res, Uji, Kyoto 6110011, Japan Ist Nazl Nutr, Unita Nutr Sperimentale, I-00178 Rome, Italy Ist Nazl NutrRome Italy I-00178 Nutr Sperimentale, I-00178 Rome, Italy Univ Rome La Sapienza, Dipartimento Genet & Biol Mol, Ctr Studio Gli AcidiNucleici, I-00185 Rome, Italy Univ Rome La Sapienza Rome Italy I-00185 diNucleici, I-00185 Rome, Italy
Titolo Testata:
PLANT JOURNAL
fascicolo: 4, volume: 25, anno: 2001,
pagine: 389 - 398
SICI:
0960-7412(200102)25:4<389:NAOTAH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHADE AVOIDANCE RESPONSES; MESSENGER-RNA STABILITY; LEUCINE ZIPPER GENES; AUTO-REGULATION; DNA-BINDING; TRANSGENIC PLANTS; TARGET GENES; CELL FATE; EXPRESSION; HOMEODOMAIN;
Keywords:
induction system; target gene; glucocorticoid receptor; DNase I footprinting; transcriptional network; HD-Zip gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Aoyama, T Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan Kyoto UnivUji Kyoto Japan 6110011 s, Uji, Kyoto 6110011, Japan
Citazione:
M. Ohgishi et al., "Negative autoregulation of the Arabidopsis homeobox gene ATHB-2", PLANT J, 25(4), 2001, pp. 389-398

Abstract

The Arabidopsis homeobox gene ATHB-2 is tightly regulated by light signals, and is thought to direct morphological changes during shade avoidance responses. To understand how ATHB-2 mediates light signals in plant morphogenesis, we investigated its transcriptional network. We constructed a gene encoding a chimeric transcription factor (HD-Zip-2-V-G) that is expected to activate target genes of ATHB-2 in a glucocorticoid-dependent manner. In transgenic Arabidopsis plants expressing HD-Zip-2-V-G, glucocorticoid treatmentactivates the ATHB-2 gene itself, independent of de novo protein synthesis. An in vitro DNase I-footprinting experiment showed that recombinant ATHB-2 protein specifically bound to an ATHB-2 promoter region. These complementary results indicate that ATHB-2 recognizes its own promoter. Consistent with the fact that ATHB-2 itself has been shown to act as a repressor, expression of the endogenous ATHB-2 gene was repressed in transgenic plants overexpressing an ATHB-2 transgene. Moreover, target-gene analysis using the HD-Zip-2-V-G suggested that ATHB-2 recognizes other HD-Zip II subfamily genes. We conclude that ATHB-2 has a negative autoregulatory loop and may be involved in a complicated transcriptional network involving paralogous genes, as is the case with animal homeobox genes.

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Documento generato il 20/01/20 alle ore 07:20:56