Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
cis-Urocanic acid does not induce the expression of immunosuppressive cytokines in murine keratinocytes
Autore:
Zak-Prelich, M; Norval, M; Venner, TJ; Bisset, Y; Walker, C; Rafferty, TS; Sauder, DN; McKenzie, RC;
Indirizzi:
Med Univ Lodz, Dept Dermatol, PL-90131 Lodz, Poland Med Univ Lodz Lodz Poland PL-90131 Dept Dermatol, PL-90131 Lodz, Poland Univ Edinburgh, Sch Med, Dept Med Microbiol, Edinburgh EH8 9AG, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH8 9AG Midlothian, Scotland Univ Edinburgh, Sch Med, Dept Dermatol, Edinburgh EH8 9AG, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH8 9AG Midlothian, Scotland Univ Toronto, Div Dermatol, Toronto, ON, Canada Univ Toronto Toronto ON Canada oronto, Div Dermatol, Toronto, ON, Canada
Titolo Testata:
PHOTOCHEMISTRY AND PHOTOBIOLOGY
fascicolo: 3, volume: 73, anno: 2001,
pagine: 238 - 244
SICI:
0031-8655(200103)73:3<238:CADNIT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; DELAYED-TYPE HYPERSENSITIVITY; CONTACT HYPERSENSITIVITY; ALLOSTIMULATORY FUNCTION; ULTRAVIOLET-RADIATION; IMMUNE SUPPRESSION; MOUSE SKIN; DNA-DAMAGE; CELL-LINES; IN-VITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: McKenzie, RC Royal Infirm Edinburgh, Dept Dermatol, Lauriston Bldg, Edinburgh EH3 9YW, Midlothian, Scotland Royal Infirm Edinburgh Lauriston Bldg Edinburgh Midlothian Scotland EH3 9YW
Citazione:
M. Zak-Prelich et al., "cis-Urocanic acid does not induce the expression of immunosuppressive cytokines in murine keratinocytes", PHOTOCHEM P, 73(3), 2001, pp. 238-244

Abstract

trans-Urocanic acid (UCA) acts as a chromophore for UV radiation in the epidermis and isomerizes to cis-UCA which then initiates some of the changes leading to UV-induced immunosuppression. The mechanism of the immunomodulation by cis-UCA is unknown at present, but one possibility is that the interaction between cis-UCA. and keratinocytes causes the release of immunosuppressive cytokines locally, To test this hypothesis, PAM-212 cells, a murine keratinocyte cell line, mere incubated with 0.10-100 mug/mL trans- and cis-UCA for 6 or 24 h, respectively. The expression of interleukin (IL)-10, transforming growth factor (TGF)-beta and tumor necrosis factor (TNF)-alpha: messenger RNA (mRNA) was then measured by reverse transcription-polymerase chain reaction in comparison with the mRNA for the house-keeping gene, beta -actin, No change or significant induction of any of the cytokine messages occurred, However, the expression of IL-IO messenger RNA (mRNA) was induced24 h after UVB irradiation (300 J/m(2)) and that of TNF-alpha mRNA occurred 6 h after treatment Kith phorbol myristate acetate, The expression of IL-10 protein was also examined by immunostaining in both PAM-212 cells and B16-F10 murine melanoma cells between 3 and 48 h after incubation with 10 and100 mug/mL cis- and trans-UCA, No alteration was seen with either isomer at either concentration. In contrast, UVB irradiation of both cell lines resulted in a marked increase in intracellular IL-10 protein at 24 and 48 h, Therefore the upregulation of the immunosuppressive cytokines, IL-10, TNF-alpha and TGF-beta in keratinocytes is unlikely to be the mechanism by which cis-UCA induces immunosuppression in mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:25:11