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Titolo:
Quantitative immunohistochemical evaluation of HER2/neu expression with HercepTest(TM) in breast carcinoma by image analysis
Autore:
Hatanaka, Y; Hashizume, K; Kamihara, Y; Itoh, H; Tsuda, H; Osamura, RY; Tani, Y;
Indirizzi:
DAKO Japan Co Ltd, Dept Immunohistochem, Kyoto 6008493, Japan DAKO Japan Co Ltd Kyoto Japan 6008493 unohistochem, Kyoto 6008493, Japan Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 25911, Japan Tokai Univ Isehara Kanagawa Japan 25911 l, Isehara, Kanagawa 25911, Japan Natl Canc Ctr, Res Inst & Hosp, Div Pathol, Tokyo 104, Japan Natl Canc Ctr Tokyo Japan 104 Inst & Hosp, Div Pathol, Tokyo 104, Japan
Titolo Testata:
PATHOLOGY INTERNATIONAL
fascicolo: 1, volume: 51, anno: 2001,
pagine: 33 - 36
SICI:
1320-5463(200101)51:1<33:QIEOHE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; NEU ONCOGENE; C-ERBB-2 ONCOPROTEIN; GENE AMPLIFICATION; CANCER; HER-2/NEU; TISSUE; OVEREXPRESSION; PROTOONCOGENE; ANTIBODIES;
Keywords:
HER2/neu protein; HercepTest(TM); image analysis; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Tani, Y DAKO Japan Co Ltd, Dept Immunohistochem, 18 Kakkyoyama,NishinotoinHigashiiru, Kyoto 6008493, Japan DAKO Japan Co Ltd 18 Kakkyoyama,Nishinotoin Higashiiru Kyoto Japan 6008493
Citazione:
Y. Hatanaka et al., "Quantitative immunohistochemical evaluation of HER2/neu expression with HercepTest(TM) in breast carcinoma by image analysis", PATHOL INT, 51(1), 2001, pp. 33-36

Abstract

HercepTest(TM) (DAKO A/S, Glostrup, Denmark) is an immunohistochemical assay that detects HER2/neu gene products, and evaluates the overexpression status of the HER2/neu protein in determining eligibility for the Trastuzumab(Herceptin(R), Genentech, San Francisco, CA, USA) therapy. However, practically, interobserver variability of the HER2/neu interpretation of the immunostained results has caused marked disagreement with regard to the intensity of tumor staining. In this study, we quantitated HER2/neu expression by image analysis, and applied this analyzing system to help to minimize interobserver variability of the interpretation of the HercepTest(TM). All the immunostained results were scored semiquantitatively on a range of 0 to 3+ in accordance with the criteria described as per the manufacturer's instructions, and quantitatively evaluated using an image analyzing system with image processing software. Among the 92 cases, 15 were scored as 3+, six were 2+, and 32 were 1+ under intraobservers agreement. When the cases were quantitated, a high correlation was shown between the signal area extracted by image analysis and the corresponding score of staining intensity with the HercepTest(TM). By converting the quantitatively extracted data into a scoring system based upon the criteria, the outcome demonstrated a strong concordance with the scoring data obtained from immunostaining. The results indicated that a quantitative scoring system performed by simple image analysis may provide to improve interobserver agreement of the interpretation of theHercepTest(TM) in clinical practice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:34:33