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Titolo:
Effects of synthetic serine protease inhibitors on proliferation and collagen synthesis of human pancreatic periacinar fibroblast-like cells
Autore:
Nakamura, F; Shintani, Y; Saotome, T; Fujiyama, Y; Bamba, T;
Indirizzi:
Shiga Univ Med Sci, Dept Internal Med, Shiga 5202192, Japan Shiga Univ MedSci Shiga Japan 5202192 nternal Med, Shiga 5202192, Japan
Titolo Testata:
PANCREAS
fascicolo: 3, volume: 22, anno: 2001,
pagine: 317 - 325
SICI:
0885-3177(200104)22:3<317:EOSSPI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING GROWTH-FACTOR; EXOCRINE FUNCTION; STELLATE CELLS; RATS; IDENTIFICATION; CERULEIN; TRYPSIN; ULTRASTRUCTURE; STIMULATION; KALLIKREIN;
Keywords:
human pancreatic periacinar fibroblast-like cells; serine protease inhibitor; cell proliferation; collagen synthesis; cytokine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Nakamura, F Shiga Univ Med Sci, Dept Internal Med, Shiga 5202192, Japan Shiga Univ Med Sci Shiga Japan 5202192 Shiga 5202192, Japan
Citazione:
F. Nakamura et al., "Effects of synthetic serine protease inhibitors on proliferation and collagen synthesis of human pancreatic periacinar fibroblast-like cells", PANCREAS, 22(3), 2001, pp. 317-325

Abstract

Protease inhibitors are currently used as therapeutic agents for chronic pancreatitis in Japan. We previously reported that human pancreatic periacinar fibroblast-like cells (hPFCs) could be cultured from isolated pancreaticacini, and those are thought to play a crucial role in pancreatic fibrosiscorrelating with platelet-derived growth factor (PDGF) and transforming growth factor beta1 (TGF-beta1) (Pancreas 1997;14: 373-82. The present study was designed to examine the effects of synthetic serine protease inhibitors(FOY-007 and FOY-305) on proliferation and collagen synthesis of hPFCs under cytokine stimulation. The cell proliferation and collagen synthesis wereevaluated using assays of [H-3]-thymidine incorporation and procollagen type I c-terminal peptide (PIP), and [C-14]-proline incorporation to de novo synthesized collagen, respectively. The cell proliferation stimulated by PDGF was inhibited by the application of FOY-007 dose dependently (1-100 muM)and FOY-305 at 100 muM. FOY-007 attenuated the collagen synthesis and PIP production stimulated by TGF-beta1 dose dependently, but FOY-305 inhibited only PIP production. Both protease inhibitors demonstrated no effect on theproliferation and collagen synthesis of hPFCs when they were not stimulated by PDGF or TGF-beta1. Thus, serine protease inhibitors act on hPFCs to diminish the effects of PDGF on proliferation and the effects of TGF-beta1 oncollagen synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:31:50