Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Constitutive c-Myb amino-terminal phosphorylation and DNA binding activityuncoupled during entry and passage through the cell cycle
Autore:
Cures, A; House, C; Kanei-Ishii, C; Kemp, B; Ramsay, RG;
Indirizzi:
Peter MacCallum Canc Inst, Trescowthick Res Labs, Melbourne, Vic 3000, Australia Peter MacCallum Canc Inst Melbourne Vic Australia 3000 ic 3000, Australia RIKEN, Tsukuba Inst, Genet Mol Lab, Tsukuba, Ibaraki 3050074, Japan RIKENTsukuba Ibaraki Japan 3050074 Lab, Tsukuba, Ibaraki 3050074, Japan
Titolo Testata:
ONCOGENE
fascicolo: 14, volume: 20, anno: 2001,
pagine: 1784 - 1792
SICI:
0950-9232(20010329)20:14<1784:CCAPAD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTO-ONCOGENE; LEUCINE-ZIPPER; MESSENGER-RNA; EXPRESSION; PROLIFERATION; ACTIVATION; PROTEINS; GENE; DIFFERENTIATION; INHIBITION;
Keywords:
c-Myb; casein kinase 2; DNA binding; phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ramsay, RG Peter MacCallum Canc Inst, Trescowthick Res Labs, 481 Little Lonsdale St, Melbourne, Vic 3000, Australia Peter MacCallum Canc Inst 481 Little Lonsdale St Melbourne Vic Australia 3000
Citazione:
A. Cures et al., "Constitutive c-Myb amino-terminal phosphorylation and DNA binding activityuncoupled during entry and passage through the cell cycle", ONCOGENE, 20(14), 2001, pp. 1784-1792

Abstract

The c-myb gene encodes a transcription factor that is central to hematopoietic cell growth, Phosphorylation of c-Myb by casein kinase 2 (CK2) at serines 11 and 12 has been variously implicated in the regulation of DNA binding. However, it is unclear when c-Myb phosphorylation at serines II and 12 occurs during the cell cycle and how this is regulated, We generated specific antisera that recognize phosphoserines II and 12 of c-Myb, C-Myb protein levels, extent of CK2 phosphorylation and DNA binding were then monitored following mitogenic stimulus and passage through the cell cycle in normal peripheral T-cells and the T leukemia cell line CCRF-CEM, We found that endogenous c-Myb is constitutively phosphorylated at serines 11 and 12, The amount of phosphorylated c-Myb correlates with DNA binding activity in cycling CEM cells but not upon entry of T-cells into the cell cycle, Exogenous expression of c-Myb with substitutions of serines 11 and 12 with glutamic acid or alanine had no effect on the transactivation of a c-Myb responsive reporter. These data strongly suggest that c-Myb is constitutively phosphorylated on serines 11 and 12 by CK2 or like activity and is not regulated during the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 08:23:54