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Titolo:
Up- and down-regulation of helix command-specific 2 (HCS2) gene expressionin the nervous system of terrestrial snail Helix lucorum
Autore:
Balaban, PM; Poteryaev, DA; Zakharov, IS; Uvarov, P; Malyshev, A; Belyavsky, AV;
Indirizzi:
Russian Acad Sci, Inst Higher Nervous Act & Neurophysiol, Moscow 117865, Russia Russian Acad Sci Moscow Russia 117865 europhysiol, Moscow 117865, Russia VA Engelhardt Mol Biol Inst, Moscow 117984, Russia VA Engelhardt Mol Biol Inst Moscow Russia 117984 , Moscow 117984, Russia
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 103, anno: 2001,
pagine: 551 - 559
SICI:
0306-4522(2001)103:2<551:UADOHC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GIANT INTERNEURONS; SENSORY NEURONS; APLYSIA; INSULIN; SENSITIZATION; NEUROPEPTIDES; FAMILY; INJURY;
Keywords:
withdrawal behavior; identified neurons; serotonin; invertebrates;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Balaban, PM Russian Acad Sci, Inst Higher Nervous Act & Neurophysiol, 5A Butlerova St,Moscow 117865, Russia Russian Acad Sci 5A Butlerova St Moscow Russia 117865 Russia
Citazione:
P.M. Balaban et al., "Up- and down-regulation of helix command-specific 2 (HCS2) gene expressionin the nervous system of terrestrial snail Helix lucorum", NEUROSCIENC, 103(2), 2001, pp. 551-559

Abstract

A novel gene named Helix command-specific 2 (HCS2) was shown to be expressed predominantly in four giant parietal interneurons involved in withdrawalbehavior of the terrestrial snail Helix lucorum L. and several single neurons in other ganglia. Decrease in spontaneous electrophysiological activityof neurons in the isolated CNS by 24 h incubation in saline with elevated Mg2+ concentration significantly decreased the number of HCS2-expressing neurons. Five short-term serotonin applications (each of 10 muM), during a 24h incubation of the nervous system in saline induced expression of the HCS2 gene in many cells in cerebral, parietal, pleural and pedal ganglia. Dopamine applications under similar conditions were not effective. Application of anisomycin or cycloheximide, known to block protein synthesis, did not prevent the induction of HCS2 expression under serotonin influence. Skin injury elicited a significant increase in the number of HCS2 expressing cells 24 h later in pleural and cerebral ganglia. Incubation of the isolated nervous system preparations for three days in culture medium elicited close to a maximum increase in number of HCS2-expressing cells. Elevation of the normal Mg2+ concentration in the culture medium significantly decreased the number of cells demonstrating HCS2 expression. Application of the cAMP activator forskolin (10 muM) increased the expression under Mg2+, indicating thatcAMP was involved in the up-regulation of HCS2. Application of thapsigargin (10 muM), known to release Ca2+ from intracellular stores, was also effective in increasing expression, suggesting participation of Ca2+ in regulation of HCS2 expression. Cellular groups expressing the HCS2 gene under different conditions seem to be functionally related since it was demonstrated earlier that some neurons constituting these clusters are involved in the withdrawal behavior and the response of the organism to stress stimuli. From these results we suggest that the HCS2 pattern of expression can be down-regulated by a decrease in synaptic activity in the nervous system, andup-regulated by external noxious inputs, as well as the application of neurotransmitters and second messengers known to be involved in the withdrawalbehavior and maintenance of isolated ganglia in culture medium. When up-regulated, the HCS2 expression appears, at least in part in neurons, to be involved in the withdrawal behavior. (C) 2001 IBRO. Published by Elsevier Science Ltd: All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:57:53