Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Unilateral dopamine depletion increases expression of the 2A subunit of the N-methyl-D-aspartate receptor in enkephalin-positive and enkephalin-negative neurons
Autore:
Ganguly, A; Keefe, KA;
Indirizzi:
Univ Utah, Dept Pharmacol & Toxicol, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 xicol, Salt Lake City, UT 84112 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 103, anno: 2001,
pagine: 405 - 412
SICI:
0306-4522(2001)103:2<405:UDDIEO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; RAT STRIATAL NEURONS; BASAL GANGLIA; GENE-EXPRESSION; PARKINSONS-DISEASE; SUBSTANCE-P; EXTRACELLULAR LEVELS; NMDA RECEPTORS; NR2A SUBUNIT; AMINO-ACIDS;
Keywords:
striatum; NR2A; 6-hydroxydopamine; Parkinson's disease; in situ hybridization histochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Keefe, KA Univ Utah, Dept Pharmacol & Toxicol, 112 Skaggs Hall, Salt Lake City, UT 84112 USA Univ Utah 112 Skaggs Hall Salt Lake City UT USA 84112 84112 USA
Citazione:
A. Ganguly e K.A. Keefe, "Unilateral dopamine depletion increases expression of the 2A subunit of the N-methyl-D-aspartate receptor in enkephalin-positive and enkephalin-negative neurons", NEUROSCIENC, 103(2), 2001, pp. 405-412

Abstract

Striatal efferent neurons receive dopamine- and glutamate-utilizing afferents. Previous studies have shown that dopamine depletion increases gene expression in striatopallidal neurons and decreases it in striatonigral neurons. Previous work has also reported increased expression of the 2A subunit of the N-methyl-D-aspartate receptor in the dopamine-depleted striatum. The purpose of this study therefore was to determine whether dopamine depletiondifferentially alters the expression of the 2A subunit of the N-methyl-D-aspartate receptor in rat striatal neurons. 6-Hydroxydopamine (8 mug/2 mul) was infused unilaterally into the medial forebrain bundle. Rats were killedthree weeks later. Double-label in situ hybridization was performed using an S-35-labeled ribonucleotide probe directed against the messenger RNA of the 2A subunit and a digoxigenin-labeled ribonucleotide probe directed towards preproenkephalin messenger RNA to mark striatopallidal neurons. Analysis of single-labeled film autoradiograms revealed a significant increase in the expression of 2A subunit messenger RNA in the ipsilateral, but not the contralateral, striatum of dopamine-depleted animals, consistent with otherstudies in the literature, Cellular analysis of 2A subunit expression indicated that as a consequence of dopamine depletion there is a significant increase in the expression of this subunit in both enkephalin-positive and enkephalin-negative neurons. From this study we conclude that dopamine depletion increases messenger RNA expression of the 2A subunit of the N-methyl-D-aspartate receptor in striatopallidal and presumed striatonigral (enkephalin-negative) neurons. Such alterations may affect the pharmacology and function of the resultant receptor, and thus alter glutamate transmission in both populations of medium spiny neurons after dopamine depletion. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 20:18:32