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Titolo:
Intraglomerular synthesis of complement C3 and its activation products in IgA nephropathy
Autore:
Abe, K; Miyazaki, M; Koji, T; Furusu, A; Shioshita, K; Tsukasaki, S; Ozono, Y; Harada, T; Sakai, H; Kohno, S;
Indirizzi:
Nagasaki Univ, Sch Med, Dept Internal Med 2, Nagasaki 8528501, Japan Nagasaki Univ Nagasaki Japan 8528501 rnal Med 2, Nagasaki 8528501, Japan Nagasaki Univ, Sch Med, Dept Histol & Cell Biol, Nagasaki 8528501, Japan Nagasaki Univ Nagasaki Japan 8528501 Cell Biol, Nagasaki 8528501, Japan Nagasaki Univ, Sch Med, Div Renal Care Unit, Nagasaki 8528501, Japan Nagasaki Univ Nagasaki Japan 8528501 Care Unit, Nagasaki 8528501, Japan Tokai Univ, Dept Nephrol, Isehara, Kanagawa 25911, Japan Tokai Univ Isehara Kanagawa Japan 25911 l, Isehara, Kanagawa 25911, Japan
Titolo Testata:
NEPHRON
fascicolo: 3, volume: 87, anno: 2001,
pagine: 231 - 239
SICI:
0028-2766(200103)87:3<231:ISOCCA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOMERULAR EPITHELIAL-CELLS; HUMAN MESANGIAL CELLS; HUMAN-ENDOTHELIAL CELLS; GENE-EXPRESSION; 3RD COMPONENT; MEMBRANOUS NEPHROPATHY; MESSENGER-RNA; C5B-9 COMPLEX; HUMAN KIDNEY; SERUM IGA;
Keywords:
complement C3; in situ hybridization; membrane attack complex; IgA nephropathy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Abe, K Nagasaki Univ, Sch Med, Dept Internal Med 2, 1-7-1 Sakamoto, Nagasaki 8528501, Japan Nagasaki Univ 1-7-1 Sakamoto Nagasaki Japan 8528501 8528501, Japan
Citazione:
K. Abe et al., "Intraglomerular synthesis of complement C3 and its activation products in IgA nephropathy", NEPHRON, 87(3), 2001, pp. 231-239

Abstract

Background: Complement activation is thought to be pathologically important in IgA nephropathy (IgAN). Although C3 deposition in the mesangium is found in IgAN, the origin of C3 is not clear. We recently demonstrated intraglomerular C3 synthesis in the human kidney; however, the activation and pathological role of locally synthesized C3 remains unclear. Here we performed nonradioactive in situ hybridization for C3 mRNA and immunohistochemistry for C3 and its activation products, such as C3d and membrane attack complex (MAC), to determine whether locally produced C3 in glomeruli was activated in IgA nephropathy. Methods: Renal samples from 14 patients with IgAN and 5with minimal change nephrotic syndrome (MCNS) were examined. Uninvolved portions of surgically removed kidneys with tumors served as normal controls. Results: C3 mRNA was not detected in glomeruli in control tissue and MCNS,but was strongly expressed in resident glomerular cells of IgAN, includingmesangial cells, glomerular epithelial cells and the cells of Bowman's capsule. Examination of serial sections disclosed that more than 70% of cells positive for C3 mRNA were also stained for C3 protein, C3d, and MAC. Doublestaining for in situ hybridization and immunohistochemistry also revealed that those C3 mRNA signals were present in intraglomerular cells positive for C3. The expression of C3 mRNA and MAC in glomeruli correlated significantly with the degree of mesangial matrix expansion. Conclusions: Our resultsdemonstrated that locally synthesized C3 is activated in the glomeruli of IgAN and that its expression correlated with the severity of mesangial matrix expansion. These findings suggest that activation of C3 may be involved in tissue injury in IgAN th rough the formation of membrane attack complex. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:28:53