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Titolo:
Generation and characterization of a PhoP homologue mutant of Neisseria meningitidis
Autore:
Johnson, CR; Newcombe, J; Thorne, S; Borde, HA; Eales-Reynolds, LJ; Gorringe, AR; Funnell, SGP; McFadden, JJ;
Indirizzi:
Univ Surrey, Sch Biol Sci, Guildford GU2 5XH, Surrey, England Univ SurreyGuildford Surrey England GU2 5XH ord GU2 5XH, Surrey, England Publ Hlth Lab Serv, Ctr Appl Microbiol & Res, Salisbury SP4 0JG, Wilts, England Publ Hlth Lab Serv Salisbury Wilts England SP4 0JG P4 0JG, Wilts, England
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 1345 - 1355
SICI:
0950-382X(200103)39:5<1345:GACOAP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMONELLA-TYPHIMURIUM; TRANSCRIPTIONAL REGULATOR; TOLERANCE RESPONSE; ESCHERICHIA-COLI; VIRULENCE GENE; PROTEIN; RESISTANCE; EXPRESSION; IRON; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: McFadden, JJ Univ Surrey, Sch Biol Sci, Guildford GU2 5XH, Surrey, EnglandUniv Surrey Guildford Surrey England GU2 5XH Surrey, England
Citazione:
C.R. Johnson et al., "Generation and characterization of a PhoP homologue mutant of Neisseria meningitidis", MOL MICROB, 39(5), 2001, pp. 1345-1355

Abstract

Two-component regulatory systems are important regulators of virulence genes in a number of bacteria. Genes encoding a two-component regulator system, with homology to the phoP/phoQ system in salmonella, were identified in the meningococcal genome. Allele replacement was used to generate a meningococcal knock-out mutant of the regulator component of this system, and its phenotype was examined. The mutant displayed many differences in protein profiles compared with wild type, consistent with it being a gene-regulatory mutation. Many of the growth characteristics of the mutant were similar to those of phoP mutants of salmonella: it was unable to grow at low concentrations of magnesium and was sensitive to defensins and other environmental stresses. Magnesium-regulated differences in protein expression were abrogated in the mutant, indicating that the meningococcal PhoP/PhoQ system may, asin salmonella, respond to changes in environmental magnesium levels. Theseresults are consistent with the PhoP homologue playing a similar role in the meningococcus to PhoP in salmonella and suggest that it may similarly beinvolved in the regulation of virulence genes in response to environmentalstimuli in the meningococcus. In support of this conclusion, we found the mutant grew was unable to grow in mouse serum and was attenuated in its ability to traverse through a layer of human epithelial cells. Identification of those genes regulated by the meningococcal PhoP may provide a route towards the identification of virulence genes in the meningococcus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:38:17