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Titolo:
Lipid phase separation correlates with activation in platelets during chilling
Autore:
Tsvetkova, NM; Walker, NJ; Crowe, JH; Field, CL; Shi, Y; Tablin, F;
Indirizzi:
Univ Calif Davis, Sch Vet Med, Sect Mol & Cellular Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 & Cellular Biol, Davis, CA 95616 USA Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 siol & Cell Biol, Davis, CA 95616 USA
Titolo Testata:
MOLECULAR MEMBRANE BIOLOGY
fascicolo: 4, volume: 17, anno: 2000,
pagine: 209 - 218
SICI:
0968-7688(200010/12)17:4<209:LPSCWA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TIME-RESOLVED FLUORESCENCE; HUMAN-BLOOD-PLATELETS; PLASMA-MEMBRANE; PHOSPHATIDYLCHOLINE BILAYERS; BIOLOGICAL-MEMBRANES; HUMAN-ERYTHROCYTES; PHOSPHOLIPASE A2; MODEL MEMBRANES; STORAGE LESION; CHOLESTEROL;
Keywords:
platelet; activation; membrane; domains; Dil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Tsvetkova, NM Univ Calif Davis, Sch Vet Med, Sect Mol & Cellular Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 ol, Davis, CA 95616 USA
Citazione:
N.M. Tsvetkova et al., "Lipid phase separation correlates with activation in platelets during chilling", MOL MEMBR B, 17(4), 2000, pp. 209-218

Abstract

When human platelets are chilled below 22 degreesC, they spontaneously activate, a phenomenon that severely limits their storage life. It has previously been proposed that there is a correlation between cold-induced plateletactivation and passage of the membranes through a liquid-crystalline to gel phase transition. Because animal models are essential for developing methods for cold storage of platelets, it is necessary to investigate such a correlation in animal platelets. In this work, horse platelets were used as amodel, and it was found that cold-induced morphological activation is related to the lipid phase transition. Using fluorescence microscopy with the lipophilic fluorescent dye 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indo cyanine perchlorate (Dil-C-18), and Fourier transform infrared spectroscopy (FTIR), it was found that lipid phase separation occurs during cooling and low temperature storage. Furthermore, removal of cholesterol from the plasma membrane also induced a phase separation, possibly between specific phospholipid classes. Steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) and trimethylammonium-DPH (TMA-DPH) were compared in cells and multilamellar vesicles (MLV) composed of platelet lipids. Cholesterol depletion led to a decrease in the fluorescence anisotropy of the two probes, which can be explained by changes in the order of the phospholipid molecules. In addition, the lipid composition and fatty acid profile of the cellular phospholipids were determined. Based of the similarities between horse and human platelets, it is suggested that horse platelets may be used as a model for studying cold-stored platelets. The results are discussed in relation to the possible role of phase separation during cell signalling.

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Documento generato il 23/01/20 alle ore 04:21:27