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Titolo:
Freeze-fracture studies on lipids and membranes
Autore:
Meyer, HW; Richter, W;
Indirizzi:
Klinikum Univ Jena, Inst Ultrastrukturforsch, D-07740 Jena, Germany Klinikum Univ Jena Jena Germany D-07740 turforsch, D-07740 Jena, Germany
Titolo Testata:
MICRON
fascicolo: 6, volume: 32, anno: 2001,
pagine: 615 - 644
SICI:
0968-4328(200108)32:6<615:FSOLAM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
X-RAY-DIFFRACTION; ETCH ELECTRON-MICROSCOPY; CONVEX-CONCAVE CURVATURES; LATERAL PHASE-SEPARATION; ACHOLEPLASMA-LAIDLAWII-B; TIGHT JUNCTION STRANDS; BILAYER-MEMBRANES; PLASMA-MEMBRANE; CELL-MEMBRANES; ACYL-CHAIN;
Keywords:
freeze-fracture electron microscopy; lipids; biomembranes; phase states; cold hydration; cold storage; ripples; curvatures; non-lamellar structures; phase separation;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
215
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, HW Klinikum Univ Jena, Inst Ultrastrukturforsch, D-07740 Jena, Germany Klinikum Univ Jena Jena Germany D-07740 D-07740 Jena, Germany
Citazione:
H.W. Meyer e W. Richter, "Freeze-fracture studies on lipids and membranes", MICRON, 32(6), 2001, pp. 615-644

Abstract

Freeze-fracture electron microscopy is especially useful for investigationof lipid structures by the advantageous fracture course within hydrophobiczones. Freezing is, on the other hand, a restriction because the structures of lamellar and non-lamellar phase states with disordered acyl chains (L-alpha H-II, cubic) are difficult to preserve. An important aspect of this method is therefore the lipid structure of phase states with ordered acyl chains (crystal, gel), and with a different degree of hydration. Freeze-fracture of pure lipid systems creates a valid representation of the structure of non-lamellar phases and of the general structure of the "lamellar" lipid bilayer, and lamellar phases with characteristic deformations (ripples, curvatures, plane sectors) can be identified. Fracture through the hydrophobicbilayer centre of biological membranes reveals characteristic protein components, the intramembraneous particles (IMPs). The lateral distribution of the IMPs is a helpful marker for fluid and rigid phase states, also withoutdeformation of the lamella. The overall history and the present state of knowledge concerning the different structures revealed by the freeze-fracture and freeze-etch techniques in lipid systems, and to a limited extent in biological membranes, is reviewed, taking into account studies from our own laboratory. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:26:00