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Titolo:
A comparison of F-actin labeling methods for light microscopy in differentplant specimens: multiple techniques supplement each other
Autore:
Van Gestel, K; Le, J; Verbelen, JP;
Indirizzi:
Univ Instelling Antwerp, Dept Biol, B-2610 Wilrijk, Antwerp, Belgium Univ Instelling Antwerp Wilrijk Antwerp Belgium B-2610 , Antwerp, Belgium
Titolo Testata:
MICRON
fascicolo: 6, volume: 32, anno: 2001,
pagine: 571 - 578
SICI:
0968-4328(200108)32:6<571:ACOFLM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
STAMEN HAIR-CELLS; ENDOMEMBRANE SHEATH; CYTOSKELETON; MICROINJECTION; FILAMENTS; DYNAMICS; NETWORK; MORPHOGENESIS; ARABIDOPSIS; PROTOPLASTS;
Keywords:
plant actin; phalloidin; glycerol; microinjection; confocal laser scanning microscopy;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Van Gestel, K Univ Instelling Antwerp, Dept Biol, Univ Pl 1, B-2610 Wilrijk, Antwerp, Belgium Univ Instelling Antwerp Univ Pl 1 Wilrijk Antwerp Belgium B-2610
Citazione:
K. Van Gestel et al., "A comparison of F-actin labeling methods for light microscopy in differentplant specimens: multiple techniques supplement each other", MICRON, 32(6), 2001, pp. 571-578

Abstract

Different detection methods for F-actin labeling were compared on a range of plant specimens: cultured cells, whole organ mounts and sectioned material. For cultured cells, microinjection of labeled phalloidin yielded the most detailed picture but careful permeation methods come close, while immunocytochemical methods always gave relatively poor detail, especially on the level of the fine filaments. For whole organ mounts and sectioned material,permeation methods and immunolocalization are the methods of choice, however never reaching the level of resolution of permeation methods in single cells. It is clear that there is no general and universal good method and multiple techniques are needed, especially when working with different specimens and with different aims. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 07:04:07