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Titolo:
Dietary zinc deficiency increases uroguanylin accumulation in rat kidney
Autore:
Cui, L; Blanchard, RK; Cousins, RJ;
Indirizzi:
Univ Florida, Dept Food Sci & Human Nutr, Gainesville, FL 32611 USA Univ Florida Gainesville FL USA 32611 man Nutr, Gainesville, FL 32611 USA Univ Florida, Ctr Nutr Sci, Gainesville, FL 32611 USA Univ Florida Gainesville FL USA 32611 Nutr Sci, Gainesville, FL 32611 USA
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 4, volume: 59, anno: 2001,
pagine: 1424 - 1431
SICI:
0085-2538(200104)59:4<1424:DZDIUA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL GUANYLATE-CYCLASE; MESSENGER-RNA EXPRESSION; ENDOGENOUS ACTIVATOR; TISSUE DISTRIBUTION; CYCLIC-GMP; PEPTIDE; CDNA; LOCALIZATION; PLASMA; PROUROGUANYLIN;
Keywords:
diarrhea; endocrine axis; renal proximal tubules; natriuretic peptide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Cousins, RJ Univ Florida, Dept Food Sci & Human Nutr, 201 FSHN,POB 110370,Gainesville, FL 32611 USA Univ Florida 201 FSHN,POB 110370 Gainesville FL USA 32611 USA
Citazione:
L. Cui et al., "Dietary zinc deficiency increases uroguanylin accumulation in rat kidney", KIDNEY INT, 59(4), 2001, pp. 1424-1431

Abstract

Background Zinc deficiency in humans produces a secretory diarrhea that iscorrected by zinc supplementation. In rats, differential mRNA display analysis has shown that intestinal uroguanylin gene expression is increased in zinc deficiency. An endocrine axis involving intestinal uroguanylin and thekidney may exist. Therefore, we conducted this study to examine whether zinc deficiency would affect uroguanylin expression in the kidney of rats. Methods. A purified diet, deficient or adequate in zinc content, was fed to rats. Preprouroguanylin mRNA was localized in kidney by in situ hybridization, and prouroguanylin/uroguanylin peptides were localized in the kidney by immunohistochemistry. Abundance was measured by Western blotting and slot blotting analyses. Results. In situ hybridization demonstrated that preprouroguanplin mRNA-expressing cells were localized in the proximal tubules, being primarily limited to the cortical-medullary junction. Zinc deficiency did not alter the abundance or distribution of the mRNA Immunohistochemistry, using a uroguanylin peptide-specific, affinity-purified antibody, demonstrated that immunoreactive uroguanylin peptide was localized to the same cells but that the staining was stronger in zinc-deficient rats. Western blotting analysis of kidney extracts showed that there was no difference in abundance of prouroguanylin between zinc adequate and deficient rats. However, slot blotting analysis demonstrated that the abundance of a tory molecular weight immunoreactive peptide, presumably uroguanylin, was higher in extracts of zinc-deficient rats. Conclusion. The results suggest that production of prouroguanylin by the kidney, in contrast to the intestine: is not influenced by dietary zinc intake, but that higher amounts of uroguanylin in kidney extracts may reflect renal processing of the hormone obtained from the systemic circulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 15:55:16