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Titolo:
Region of herpes simplex virus type 1 latency-associated transcript sufficient for wild-type spontaneous reactivation promotes cell survival in tissue culture
Autore:
Inman, M; Perng, GC; Henderson, G; Ghiasi, H; Nesburn, AB; Wechsler, SL; Jones, C;
Indirizzi:
Univ Nebraska, Dept Vet & Biomed Sci, Lincoln, NE 68583 USA Univ NebraskaLincoln NE USA 68583 et & Biomed Sci, Lincoln, NE 68583 USA Cedars Sinai Med Ctr, Burns & Allen Res Inst, Ophthalmol Res Labs, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr Los Angeles CA USA 90048 , Los Angeles, CA 90048 USA Univ Calif Los Angeles, Sch Med, Dept Ophthalmol, Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 8, volume: 75, anno: 2001,
pagine: 3636 - 3646
SICI:
0022-538X(200104)75:8<3636:ROHSVT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRIGEMINAL GANGLIA; BOVINE HERPESVIRUS-1; NEURONAL APOPTOSIS; SODIUM-BUTYRATE; LAT GENE; SYMPATHETIC NEURONS; BLOCKS APOPTOSIS; INFECTED MICE; DNA-DAMAGE; DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Jones, C Univ Nebraska, Dept Vet & Biomed Sci, Fair St & E Campus Loop, Lincoln, NE68583 USA Univ Nebraska Fair St & E Campus Loop Lincoln NE USA 68583 83 USA
Citazione:
M. Inman et al., "Region of herpes simplex virus type 1 latency-associated transcript sufficient for wild-type spontaneous reactivation promotes cell survival in tissue culture", J VIROLOGY, 75(8), 2001, pp. 3636-3646

Abstract

The latency-associated transcript (LAT) is the only abundant herpes simplex virus type 1 (HSV-1) transcript expressed during latency. In the rabbit eye model, LAT null mutants do not reactivate efficiently from latency. We recently demonstrated that the LAT null mutant dLAT2903 induces increased levels of apoptosis in trigeminal ganglia of infected rabbits compared to LAT(+) strains (G.-C. Perng, C. Jones, J. Ciacci-Zarella, M. Stone, G. Henderson, A. Yokht, S. M. Slanina, F. M. Hoffman, H. Ghiasi, A. B. Nesburn, and C. S. Wechsler, Science 287:1500-1503, 2000). The same study also demonstrated that a plasmid expressing LAT nucleotides 301 to 2659 enhanced cell survival of transfected cells after induction of apoptosis. Consequently, we hypothesized that LAT enhances spontaneous reactivation in part, because it promotes survival of infected neurons. Here we report on the ability of plasmids expressing different portions of the 5' end of LAT to promote cell survival after induction of apoptosis, A plasmid expressing the first 1.5 kb of LAT (LAT nucleotides 1 to 1499) promoted cell survival in neuro-2A (mouseneuronal) and CV-1 (monkey fibroblast) cells, A plasmid expressing just the first 811 nucleotides of LAT promoted cell survival less efficiently. Plasmids expressing the first 661 nucleotides or less of LAT did not promote cell survival. We previously showed that a mutant expressing just the first 1.5 kb of LAT has wild-type spontaneous reactivation in rabbits, and a mutant expressing just the first 811 nucleotides of LAT has a reactivation frequency higher than that of dLAT2903 but lower than that of wild-type virus. In addition, mutants reported here for the first time, expressing just the first 661 or 76 nucleotides of LAT, had spontaneous reactivation indistinguishable from that of the LAT null mutant dLAT2903. In summary, these studies provide evidence that there is a functional relationship between the ability of LAT to promote cell survival and its ability to enhance spontaneous reactivation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:00:40