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Titolo:
Use of dipstick tests for the rapid diagnosis of malaria in nonimmune travelers
Autore:
Jelinek, T; Grobusch, MP; Nothdurft, HD;
Indirizzi:
Univ Munich, Dept Trop Med & Infect Dis, D-80802 Munich, Germany Univ Munich Munich Germany D-80802 & Infect Dis, D-80802 Munich, Germany Humboldt Univ, Med Clin Infect Dis, Berlin, Germany Humboldt Univ BerlinGermany Univ, Med Clin Infect Dis, Berlin, Germany
Titolo Testata:
JOURNAL OF TRAVEL MEDICINE
fascicolo: 4, volume: 7, anno: 2000,
pagine: 175 - 179
SICI:
1195-1982(200007/08)7:4<175:UODTFT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMODIUM-FALCIPARUM MALARIA; POLYMERASE-CHAIN-REACTION; ANTIGEN-CAPTURE ASSAY; PARASIGHT(TM)-F TEST; RHEUMATOID-FACTOR; SENSITIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Jelinek, T Univ Munich, Dept Trop Med & Infect Dis, Leopoldstr 5, D-80802 Munich, Germany Univ Munich Leopoldstr 5 Munich Germany D-80802 unich, Germany
Citazione:
T. Jelinek et al., "Use of dipstick tests for the rapid diagnosis of malaria in nonimmune travelers", J TRAVEL M, 7(4), 2000, pp. 175-179

Abstract

Background: Swift diagnosis of falciparum mala ria in nonendemic areas is frequently com plicated by lack of experience on the side of involved laboratory personnel. Diagnostic tools based on the dipstick principle for the detection of plasmodial histidine-rich protein 2 (HRP-2) (ICT Malaria P.f. (R)) and parasite-specific lactate-dehydrogenase (pLDH) (OptiMal (R)), respectively, have become available for the qualitative detection of falciparum malaria. Methods: In order to evaluate currently available assays, a series of studies was conducted: sensitivity and specificity were evaluated by investigation of specimens from 231 febrile returnees from endemic areas, cross reactivity in patients with rheumatoid factor (RF) was assessed among 92 patients from a rheumatology unit, and the quality of dipstick self-use by febriletravelers was tested in Kenya. Results: Whereas the test kit based on the detection of HRP-2 performed with a sensitivity of 92.5% and a specificity of 98.3%, the kit for the detection of pLDH showed a sensitivity of 88.5% and a specificity of 99.4%. Cross-reactions with sera positive for rheumatoid factor occurred in 6.6% with the ICT Malaria P.f.(R), and in 3.3% with the OptiMal (R) test. Only ICT Malaria P.f.(R) was tested for quality of self-use among travelers. This dipstick assay was performed successfully by 67 patients (68.4%), but 31 (31.6%) were unable to obtain a result. Conclusion: Dipstick tests have the potential of enhancing speed and accuracy of the diagnosis of falciparum malaria, especially if nonspecialized laboratories are involved. However, microscopical testing remains mandatory in every single patient with the possible diagnosis of malaria. Self-use of dipstick tests for malaria diagnosis by travelers should only be recommended after appropriate instruction and training, including a successful performance of the test procedure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:08:33