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Titolo:
Antiestrogenicity of clarified slurry oil and two crude oils in a human breast-cancer cell assay
Autore:
Arcaro, KF; Gierthy, JF; Mackerer, CR;
Indirizzi:
SUNY Albany, Sch Publ Hlth, Dept Environm Hlth & Toxicol, Rensselaer, NY 12144 USA SUNY Albany Rensselaer NY USA 12144 h & Toxicol, Rensselaer, NY 12144 USA New York State Dept Hlth, Wadsworth Ctr Labs & Res, Albany, NY 12201 USA New York State Dept Hlth Albany NY USA 12201 & Res, Albany, NY 12201 USA Mobil Business Resources Corp, Paulsboro, NJ USA Mobil Business Resources Corp Paulsboro NJ USA s Corp, Paulsboro, NJ USA
Titolo Testata:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
fascicolo: 7, volume: 62, anno: 2001,
pagine: 505 - 521
SICI:
1528-7394(20010406)62:7<505:AOCSOA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPMENTAL TOXICITY; IN-VITRO; ESTROGEN METABOLISM; GENE-EXPRESSION; MCF-7 CELLS; P450 1A1; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; RATS; HEALTH; 17-BETA-ESTRADIOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Arcaro, KF SUNY Albany, Sch Publ Hlth, Dept Environm Hlth & Toxicol, East Campus B 334, Rensselaer, NY 12144 USA SUNY Albany East Campus B 334 Rensselaer NY USA 12144 12144 USA
Citazione:
K.F. Arcaro et al., "Antiestrogenicity of clarified slurry oil and two crude oils in a human breast-cancer cell assay", J TOX E H A, 62(7), 2001, pp. 505-521

Abstract

Exposure to crude oil and certain petroleum products can be a serious health hazard. Clarified slurry oil (CSO) is a complex mixture of hydrocarbons derived from the processing of crude oil, and is a known systemic and developmental toxicant. mutagen, and carcinogen. In the present study, CSO and two crude oils, Belridge heavy crude oil (BHCO) and Lost Hills light crude oil (LHLCO), were examined for their estrogenic and antiestrogenic properties in a human breast-cancer cell (MCF-7) assay. The MCF-7 focus assay is based on postconfluent cell growth and tissue restructuring, measured as the postconfluent development of multicellular nodules or loci. The mutagenicityindices of BHCO and LHLCO also were determined in a modified Ames Salmonella assay. Oil samples were prepared in dimethyl sulfoxide, resulting in extraction of virtually all of the aromatic compounds including the sulfur- and nitrogen-substituted three- to seven-ring polycyclic aromatic compounds comprising 62.2% of the CSO, 9% of the BHCO, and 2% of the LHLCO by total weight. None of the three samples was estrogenic in the MCF-7 locus assay. Incontrast, all of the samples were antiestrogenic; that is, they inhibited the development of foci induced by 1 nM 17 beta -estradiol (E-2). The potencies of the oil samples for both antiestrogenicity and mutagenicity were correlated with the percent of polycyclic aromatic compounds they contained. Two potential mechanisms for the observed antiestrogenicity were examined. Radiometric analysis of the catabolism of [H-3]E-2 in MCF-7 cell cultures demonstrated that all three samples increased catabolism of E-2. Results from a whole-cell estrogen-receptor (ER) binding assay suggested that metabolites of compounds in the oil samples might have competed with [H-3]E-2 for ER in the MCF-7 cultures. Thus the antiestrogenicity ui the oil samples may occur through at least two mechanisms, increased catabolism of E-2 and antagonistic binding to ER.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:29:31