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Titolo:
Characterization of 5-HT2A receptor desensitization and the effect of cycloheximide on it in C6 cells
Autore:
Miyoshi, I; Kagaya, A; Kohchi, C; Morinobu, S; Yamawaki, S;
Indirizzi:
Hiroshima Univ, Sch Med, Dept Psychiat & Neurosci, Hiroshima, Japan Hiroshima Univ Hiroshima Japan pt Psychiat & Neurosci, Hiroshima, Japan Japan Sci & Technol Corp, Core Res & Evaluat Sci & Technol, Tokyo, Japan Japan Sci & Technol Corp Tokyo Japan valuat Sci & Technol, Tokyo, Japan Hiroshima Univ, Radioisotope Ctr, Hiroshima, Japan Hiroshima Univ Hiroshima Japan Univ, Radioisotope Ctr, Hiroshima, Japan
Titolo Testata:
JOURNAL OF NEURAL TRANSMISSION
fascicolo: 3, volume: 108, anno: 2001,
pagine: 249 - 260
SICI:
0300-9564(2001)108:3<249:CO5RDA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATED PHOSPHOINOSITIDE HYDROLYSIS; INTRACELLULAR CALCIUM MOBILIZATION; SMOOTH-MUSCLE CELLS; DOWN-REGULATION; GLIOMA-CELLS; DEPRESSED-PATIENTS; BLOOD-PLATELETS; 5-HYDROXYTRYPTAMINE(2A) RECEPTOR; PHARMACOLOGICAL CHARACTERIZATION; RAPID DESENSITIZATION;
Keywords:
5-MT2A receptor; desensitization; Ca; cycloheximide; C6 glioma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Yamawaki, S Hiroshima Univ, Sch Med, Dept Psychiat & Neurosci, Hiroshima, Japan Hiroshima Univ Hiroshima Japan & Neurosci, Hiroshima, Japan
Citazione:
I. Miyoshi et al., "Characterization of 5-HT2A receptor desensitization and the effect of cycloheximide on it in C6 cells", J NEURAL TR, 108(3), 2001, pp. 249-260

Abstract

Effect of prolonged pretreatment with serotonin (5-HT) on 5-HT2A receptor desensitization was examined by the measurement of intracellular calcium ([Ca2+](i)) mobilization in C6 cells. 5-HT-induced desensitization of [Ca2+](i) mobilization was in a time and dose dependent manner and reached a plateau after 3 hr. After 1 and 3 hr 5-HT pretreatment, 5-HT concentration in the medium little changed. 5-HT pretreatment with cycloheximide, a protein synthesis inhibitor, produced an enhancement of the desensitization for 3 and6 hr pretreatment. However, 5-HT pretreatment for 3 and 6 hr caused no marked change in the 5-HT2A receptor mRNA level or G alphaq/11 protein in thisstudy, suggesting that 5-HT may decrease 5-HT-induced [Ca2+](i) mobilization independent of 5-HT2A receptor mRNA or G-proteins. Endothelin-l-induced [Ca2+](i) mobilization did not alter after 5-HT and/or cycloheximide pretreatment. These results showed that activation of the 5-HT2A receptor inducedhomologous desensitization and pretreatment with 5-HT and/or cycloheximidedid not change the efficacy of the second messenger pathway from Gq to a [Ca2+](i) rise.

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Documento generato il 25/01/20 alle ore 18:41:11