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Titolo:
Keratohyalin-like granules in lizard epidermis: Evidence from cytochemical, autoradiographic, and microanalytic studies
Autore:
Alibardi, L;
Indirizzi:
Univ Bologna, Dipartimento Biol Evoluzionist & Sperimentale, Bologna, Italy Univ Bologna Bologna Italy Evoluzionist & Sperimentale, Bologna, Italy
Titolo Testata:
JOURNAL OF MORPHOLOGY
fascicolo: 1, volume: 248, anno: 2001,
pagine: 64 - 79
SICI:
0362-2525(200104)248:1<64:KGILEE>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TURTLE EMYDURA-MACQUARII; X-RAY MICROANALYSIS; NATRIX-PISCATOR; STRUCTURAL ORGANIZATION; REGENERATING EPIDERMIS; PROTEIN HISTOCHEMISTRY; EMBRYONIC-DEVELOPMENT; QUANTITATIVE ASSAY; ANOLIS-LINEATOPUS; PODARCIS-MURALIS;
Keywords:
lizard; epidermis; keratohyalin; immunohistochemistry; autoradiography; x-ray analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Alibardi, L Dipartimento Biol, Via Selmi 3, I-40126 Bologna, Italy Dipartimento Biol Via Selmi 3 Bologna Italy I-40126 na, Italy
Citazione:
L. Alibardi, "Keratohyalin-like granules in lizard epidermis: Evidence from cytochemical, autoradiographic, and microanalytic studies", J MORPH, 248(1), 2001, pp. 64-79

Abstract

Epidermal sloughing in lizards is determined by the formation of an intraepithelial shedding complex in which keratohyalin-like granules are formed. The chemical nature of these granules is unknown, as is their role in keratinization. The goal of this study was to test whether they contain some amino acids similar to those found in mammalian keratohyalin. The embryonic and regenerating epidermis of lizards are useful systems to study the formation of these granules. Histochemically keratohyalin-like granules react to histidine and contain some sulfhydryl groups (cysteine). X-ray microanalysisshows that these granules contain sulfur and often phosphorus, two elements also present in the mature clear, oberhautchen, and beta layer. Instead the mesos, alpha, and lacunar layers contain only sulfur. Most sulfur is probably in a disulfide-bonded form, particularly in mature cells of the shedding complex, in large keratohyalin-like granules, and in the beta-keratin layer. Early differentiating beta-keratin cells have the maximal incorporation of tritiated proline, whereas tritiated arginine is slightly more concentrated in the basal layer of the epidermis. A high uptake of tritiated histidine is observed mainly in keratohyalin-like granules of the clear layer, but also in the oberhautchen layer and forming the alpha-lacunar layer. Immunogold electron microscopy shows that keratohyalin-like granules do not localize keratin but are embedded within a keratin network. These results suggest that keratohyalin-like granules of lizards, like mammalian keratohyalin, contain some sulfur-rich and histidine-rich proteins. These granules participate in the process of hardening of the clear layer that molds the spinulae of the deeper oberhautchen to form the superficial microornamentation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:12:13