Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ex vivo-expanded hematopoietic cell graft recipients exhibit T cell repertoire diversity similar to that seen after conventional stem cell transplants
Autore:
Gokmen, E; Bachier, C; Raaphorst, FM; Muller, T; Armstrong, D; Lemaistre, CF; Teale, JM;
Indirizzi:
Univ Texas, Hlth Sci Ctr, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 th Sci Ctr, San Antonio, TX 78229 USA S Texas Canc Inst, San Antonio, TX 78229 USA S Texas Canc Inst San Antonio TX USA 78229 nst, San Antonio, TX 78229 USA VU Acad Hosp, NL-1081 HV Amsterdam, Netherlands VU Acad Hosp Amsterdam Netherlands NL-1081 HV HV Amsterdam, Netherlands Aastrom Biosci Inc, Ann Arbor, MI 48105 USA Aastrom Biosci Inc Ann Arbor MI USA 48105 ci Inc, Ann Arbor, MI 48105 USA
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 1, volume: 10, anno: 2001,
pagine: 53 - 66
SICI:
1525-8165(200102)10:1<53:EVHCGR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; HIGH-DOSE CHEMOTHERAPY; BLOOD PROGENITOR CELLS; PERIPHERAL-BLOOD; IMMUNE RECONSTITUTION; BREAST-CANCER; AUTOLOGOUS TRANSPLANTATION; MONONUCLEAR-CELLS; EXVIVO EXPANSION; CLONAL EXPANSION;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Teale, JM Univ Texas, Hlth Sci Ctr, 7703 Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas 7703 Floyd Curl Dr San Antonio TX USA 78229 78229 USA
Citazione:
E. Gokmen et al., "Ex vivo-expanded hematopoietic cell graft recipients exhibit T cell repertoire diversity similar to that seen after conventional stem cell transplants", J HEMATH ST, 10(1), 2001, pp. 53-66

Abstract

The feasibility of using ex vivo-expanded hematopoietic progenitor cells to reconstitute hematopoiesis after high-dose chemotherapy is presently being examined. Early studies have shown that myeloid and erythroid hematopoiesis can be successfully reconstituted after high-dose chemotherapy and ex vivo-expanded hematopoietic cell transplantation. The lymphoid reconstitution, however, has not been addressed previously. In this study, we examined the diversity of the T cell receptor V beta chain (TCRBV) repertoires in 5 breast cancer patients who were transplanted with ex vivo-expanded bone marrow mononuclear cells as the only source of hematopoietic graft. Using the TCRBV third complementarity determining region (CDR3) fingerprinting methodology, it is shown that CD4(+) and CD8(+) T cell subsets after ex vivo-expanded hematopoietic cell graft transplants exhibit TCRBV diversities that are similar in complexity when compared to those seen after conventional autologous peripheral blood stem cell transplants (PBSCT). No apparent differencein the extent of CDR3 diversity was found between ex vivo expanded and conventional autologous PBSCT recipients when the CD4(+) and CD8(+) subsets were further separated into CD45RA(+) "naive" and CD45RO(+) "memory" subsets. The diversity of the CD45RA(+) naive subsets was as complex as that of theCD45RO(+) memory subsets. These results indicate that T cell repertoire diversification is not further compromised when ex vivo-expanded hematopoietic cells are used instead of autologous peripheral blood stem cells as the only source of graft.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 00:22:34