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Titolo:
Colocalization of GABA and glycine in the ventral nucleus of the lateral lemniscus in rat: An in situ hybridization and semiquantitative immunocytochemical study
Autore:
Riquelme, R; Saldana, E; Osen, KK; Ottersen, OP; Merchan, MA;
Indirizzi:
Univ Salamanca, Lab Neurobiol Hearing, Dept Cell Biol & Pathol, E-37007 Salamanca, Spain Univ Salamanca Salamanca Spain E-37007 Pathol, E-37007 Salamanca, Spain Inst Neurosci Castilla & Leon INCyL, E-37007 Salamanca, Spain Inst Neurosci Castilla & Leon INCyL Salamanca Spain E-37007 manca, Spain Univ Oslo, Inst Basic Med Sci, Dept Anat, N-0317 Oslo, Norway Univ Oslo Oslo Norway N-0317 sic Med Sci, Dept Anat, N-0317 Oslo, Norway
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 4, volume: 432, anno: 2001,
pagine: 409 - 424
SICI:
0021-9967(20010416)432:4<409:COGAGI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTEROVENTRAL COCHLEAR NUCLEUS; INFERIOR COLLICULUS NEURONS; SUPERIOR OLIVARY COMPLEX; STEM AUDITORY NUCLEI; BUSHY CELL AXONS; BIG BROWN BAT; BRAIN-STEM; SYNAPTIC VESICLES; GABAERGIC NEURONS; VESICULAR GABA;
Keywords:
auditory brainstem; glutamic acid decarboxylase (GAD65); inhibitory amino acids; monaural; optical densitometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Merchan, MA Univ Salamanca, Fac Med, Dept Biol Celular & Patol, Lab Neurobiol Audic, E-37007 Salamanca, Spain Univ Salamanca Salamanca Spain E-37007 7007 Salamanca, Spain
Citazione:
R. Riquelme et al., "Colocalization of GABA and glycine in the ventral nucleus of the lateral lemniscus in rat: An in situ hybridization and semiquantitative immunocytochemical study", J COMP NEUR, 432(4), 2001, pp. 409-424

Abstract

We have studied by in situ hybridization for GAD65 mRNA in thick sections and by semiquantitative postembedding immunocytochemistry in consecutive semithin sections, the expression of gamma -aminobutyric acid (GABA) and glycine in cell bodies and axosomatic puncta of the rat ventral nucleus of the lateral lemniscus (VNLL), a prominent monaural brainstem auditory structure. The in situ hybridization and the densitometric analysis of the immunostaining suggest that the rat VNLL contains two main populations of neurons. Approximately one-third of neurons are unstained with either technique and are presumably excitatory; their cell bodies are enveloped by a large numberof glycine-immunoreactive puncta. Most if not all of the remaining two-thirds colocalize GABA and glycine and are assumed to be inhibitory. These twopopulations show a complementary distribution within the VNLL, with inhibitory neurons located mainly ventrally and excitatory neurons dorsally. In scatterplots of gray values measured from cell bodies, the double-labeled cells appear to form a single cluster in terms of their staining intensities for the two transmitter candidates. However, this cluster may have to be further subdivided because cells with extreme GABA/glycine ratios differ fromthose with average ratios with respect to location or size. The VNLL seemsunique among auditory structures by its large number of neurons that colocalize GABA and glycine. Although the functional significance of this colocalization remains unknown, our results suggest that the VNLL exerts convergent excitatory and inhibitory influences over the inferior colliculus, whichmay underlie the timing processing in the auditory midbrain. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 20/09/20 alle ore 07:34:50