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Titolo:
Measurement of one-bond H-1(alpha)-C-13(alpha) couplings in backbone-labelled proteins
Autore:
Giesen, AW; Bae, LC; Barrett, CL; Chyba, JA; Chaykovsky, MM; Cheng, MC; Murray, JH; Oliver, EJ; Sullivan, SM; Brown, JM; Dahlquist, FW; Homans, SW;
Indirizzi:
Univ Oregon, Dept Chem, Eugene, OR 97403 USA Univ Oregon Eugene OR USA 97403 v Oregon, Dept Chem, Eugene, OR 97403 USA Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England Martek Biosci, Columbia, MD 21045 USA Martek Biosci Columbia MD USA 21045Martek Biosci, Columbia, MD 21045 USA
Titolo Testata:
JOURNAL OF BIOMOLECULAR NMR
fascicolo: 3, volume: 19, anno: 2001,
pagine: 255 - 260
SICI:
0925-2738(200103)19:3<255:MOOHCI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESIDUAL DIPOLAR COUPLINGS; LIQUID-CRYSTALLINE MEDIUM; 2-DIMENSIONAL NMR-SPECTRA; HIGH-RESOLUTION NMR; MACROMOLECULES; SENSITIVITY; DISTANCES; PHASE; RANGE;
Keywords:
backbone-labelling; one-bond couplings; ubiquitin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Homans, SW Univ Oregon, Dept Chem, Eugene, OR 97403 USA Univ Oregon Eugene OR USA 97403 ept Chem, Eugene, OR 97403 USA
Citazione:
A.W. Giesen et al., "Measurement of one-bond H-1(alpha)-C-13(alpha) couplings in backbone-labelled proteins", J BIOM NMR, 19(3), 2001, pp. 255-260

Abstract

NMR dipole-dipole couplings between protein backbone nuclei (H-1(alpha), C-13(alpha), N-15, H-1(N),C-13') offer enormous scope for the rapid determination of protein global folds. Here, we show that measurement of one-bond splittings in the protein backbone is facilitated by use of protein that is selectively isotopically enriched only in the backbone atoms. In particular, H-1(alpha)-C-13(alpha) couplings can be measured simply and with high sensitivity by use of conventional heteronuclear single quantum correlation (HSQC) techniques.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 09:21:44