Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
In vivo biocompatibility of carbodiimide-crosslinked collagen matrices: Effects of crosslink density, heparin immobilization, and bFGF loading
Autore:
van Wachem, PB; Plantinga, JA; Wissink, MJB; Beernink, R; Poot, AA; Engbers, GHM; Beugeling, T; van Aken, WG; Feijen, J; van Luyn, MJA;
Indirizzi:
Univ Groningen, Fac Med Sci, Univ Hosp, Lab Tissue Engn, NL-9713 GZ Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9713 GZ Z Groningen, Netherlands Univ Twente, Dept Chem Technol, Inst Biomed Technol, Polymer Chem & BiomatGrp, NL-7500 AE Enschede, Netherlands Univ Twente Enschede Netherlands NL-7500 AE 500 AE Enschede, Netherlands
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 3, volume: 55, anno: 2001,
pagine: 368 - 378
SICI:
0021-9304(20010605)55:3<368:IVBOCC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DERMAL SHEEP COLLAGEN; ENDOTHELIAL-CELL-PROLIFERATION; TISSUE REGENERATING CAPACITY; FIXED PERICARDIUM; GROWTH-FACTOR; RELEASE; CYTOTOXICITY; LINKING; DEVICES; REPAIR;
Keywords:
vascular graft; collagen; biocompatibility; heparin; bFGF; endothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: van Wachem, PB Univ Groningen, Fac Med Sci, Univ Hosp, Lab Tissue Engn, Entrance 25,Hanzeplein 1, NL-9713 GZ Groningen, Netherlands Univ Groningen Entrance 25,Hanzeplein 1 Groningen Netherlands NL-9713 GZ
Citazione:
P.B. van Wachem et al., "In vivo biocompatibility of carbodiimide-crosslinked collagen matrices: Effects of crosslink density, heparin immobilization, and bFGF loading", J BIOMED MR, 55(3), 2001, pp. 368-378

Abstract

Collagen matrices, crosslinked using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (E) and N-hydroxvsuccinimide (N), were previously developed as a substrate for endothelial cell seeding of small-diameter vascular grafts. In the present study, the biocompatibility of various EN-crosslinked collagen matrices was evaluated following subcutaneous implantation in rats for periods up to 20 weeks. The effects of the crosslink density, referred to as the number of free primary amino groups per 1,000 amino acid residues (EN10, EN14, EN18, or EN22), the amount of heparin immobilized to EN14, and the effect of preloading heparinized EN14 with basic fibroblast growth factor (bFGF) on the induced tissue reaction were studied. EN-crosslinked collagen was biocompatible at both early and late time intervals, and matrices with high crosslink densities (i.e., EN14, EN10) especially demonstrated a significantly decreased antigenic response when compared to non-crosslinked collagen. Furthermore, increased crosslinking resulted in a decreased degradation rate. Immobilization of heparin onto EN14 resulted in a similar to EN14 (thus without heparin) or somewhat reduced tissue reaction, but fibrin formation and vascularization were increased with increasing quantities of immobilized heparin. Matrices preloaded with bFGF also demonstrated good biocompatibility, especially in combination with higher amounts of immobilized heparin. The latter matrices [EN14 with high heparin and bFGF, thus EN14-H (0.4)F and EN14-H(1.0)F] demonstrated significantly increased vascularizationfor periods up to 3 weeks. Neither heparin immobilization nor bFGF preloading induced an increased antigenic response. It is concluded that the results of this study justify further evaluation of bFGF preloaded, heparin immobilized EN14 collagen, as a matrix for endothelial cell seeding in experimental animals. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:52:11