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Titolo:
Effects of cationic liposome-encapsulated doxycycline on experimental Chlamydia trachomatis genital infection in mice
Autore:
Sangare, L; Morisset, R; Gaboury, L; Ravaoarinoro, M;
Indirizzi:
CHUM Hotel Dieu, Dept Microbiol & Infect, Montreal, PQ H2W 1T8, Canada CHUM Hotel Dieu Montreal PQ Canada H2W 1T8 , Montreal, PQ H2W 1T8, Canada CHUM Hotel Dieu, Dept Pathol, Montreal, PQ H2W 1T8, Canada CHUM Hotel Dieu Montreal PQ Canada H2W 1T8 , Montreal, PQ H2W 1T8, Canada
Titolo Testata:
Journal of antimicrobial chemotherapy
fascicolo: 3, volume: 47, anno: 2001,
pagine: 323 - 331
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRACT INFECTION; MOUSE MODEL; IN-VITRO; TETRACYCLINE; RESISTANT; INFERTILITY; SALPINGITIS; THERAPY; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ravaoarinoro, M CHUM Hotel Dieu, Dept Microbiol & Infect, 3840 St Urban St, Montreal, PQ H2W 1T8, Canada CHUM Hotel Dieu 3840 St Urban St Montreal PQCanada H2W 1T8
Citazione:
L. Sangare et al., "Effects of cationic liposome-encapsulated doxycycline on experimental Chlamydia trachomatis genital infection in mice", J ANTIMICRO, 47(3), 2001, pp. 323-331

Abstract

In a previous study, we demonstrated that cationic liposome-encapsulated doxycycline (CaL-Dox) was two-fold more effective than free doxycycline against Chlamydia trachomatis in vitro. Here, we evaluated the effects of two CaL-Dox regimens in comparison with unencapsulated doxycycline on the courseof chlamydial genital infection in mice. Progesterone-treated, female CF-1mice were challenged intravaginally with 1.2 x 10(5) inclusion-forming units (ifu) of C. trachomatis. Two days post-infection, the animals were divided into four treatment groups for im injection of doxycycline at 10 mg/kg body weight bd for 3 (3 Dox) or 7 days (7 Dox), or of CaL-Dox at the same dose level for 3 (3 CaL-Dox) or 7 days (7 CaL-Dox) consecutively. An infectedfifth group served as a control and was given an empty Cat preparation. C.trachomatis were isolated after five blind passages from 82% of infected control mice, 61.4% of 3 Dox, 52.2% of 3 CaL-Dox, 29% of 7 Dox and 20% of 7 CaL-Dox animals. Histopathological reactions were found in various tissues of the genital tract in 79.5% of infected control mice, 80.9% of 3 Dox, 65.2% of 3 CaL-Dox, 47.1% of 7 Dox and 25.7% of 7 CaL-Dox animals. Total antichlamydial antibody titres were lower in 7 CaL-Dox mice than in all the other groups(P < 0.005). The results showed that progesterone-treated CF-1 miceare suitable for investigation of both lower and upper genital tract infection with a lymphogranuloma venereum biovar strain of C. trachomatis. Neither 7 CaL-Dox nor 3 CaL-Dox treatment was more effective than unencapsulated7 Dox doses in the bacteriological cure of chlamydial genital infection inmice. However; 7 CaL-Dox prevented tissue damage in the genital tract significantly more than all the other regimens (P < 0.05). These results suggest that liposome-encapsulated doxycycline, particularly CaL-Dox, may have potential for the clinical treatment of chlamydial infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:08:58