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Titolo:
Localization and pharmacological characterization of voltage dependent calcium channels in cultured neocortical neurons
Autore:
Timmermann, DB; Lund, TM; Belhage, B; Schousboe, A;
Indirizzi:
Royal Danish Sch Pharm, Dept Pharmacol, Neurosci Res Ctr, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Copenhagen Denmark DK-2100 00 Copenhagen, Denmark
Titolo Testata:
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
fascicolo: 1, volume: 19, anno: 2001,
pagine: 1 - 10
SICI:
0736-5748(200102)19:1<1:LAPCOV>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL PYRAMIDAL NEURONS; CEREBELLAR GRANULE CELLS; MOUSE CEREBRAL-CORTEX; CA2+ CHANNELS; N-TYPE; SUBCELLULAR-DISTRIBUTION; NEUROTRANSMITTER RELEASE; BIOCHEMICAL-PROPERTIES; FUNCTIONAL EXPRESSION; ALPHA(1A) SUBUNITS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Schousboe, A Royal Danish Sch Pharm, Dept Pharmacol, Neurosci Res Ctr, Univ Sparken 2, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Univ Sparken 2 Copenhagen Denmark DK-2100
Citazione:
D.B. Timmermann et al., "Localization and pharmacological characterization of voltage dependent calcium channels in cultured neocortical neurons", INT J DEV N, 19(1), 2001, pp. 1-10

Abstract

The physiological significance and subcellular distribution of voltage dependent calcium channels was defined using calcium channel blockers to inhibit potassium induced rises in cytosolic calcium concentration in cultured mouse neocortical neurons. The cytosolic calcium concentration was measured using the fluorescent calcium chelator fura-2. The types of calcium channels present at the synaptic terminal were determined by the inhibitory actionof calcium channel blockers on potassium-induced [H-3]GABA release in the same cell preparation. L-, N-, P-, Q- and R-/T-type voltage dependent calcium channels were differentially distributed in somata, neurites and nerve terminals, omega -conotoxin MVIIC (omega -CgTx MVIIC) inhibited approximately 40% of the Ca2+-rise in both somata and neurites and 60% of the potassiuminduced [H-3]GABA release, indicating that the Q-type channel is the quantitatively most important voltage dependent calcium channel in all parts of the neuron. After treatment with thapsigargin the increase in cytosolic calcium was halved, indicating that calcium release from thapsigargin sensitive intracellular calcium stores is an important component of the potassium induced rise in cytosolic calcium concentration. The results of this investigation demonstrate that pharmacologically distinct types of voltage dependent calcium channels are differentially localized in cell bodies, neurites and nerve terminals of mouse cortical neurons but that the Q-type calcium channel appears to predominate in all compartments. (C) 2001 ISDN. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:06:17