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Titolo:
Complete nucleotide sequence and analysis of the locus of enterocyte effacement from rabbit diarrheagenic Escherichia coli RDEC-1
Autore:
Zhu, CR; Agin, TS; Elliott, SJ; Johnson, LA; Thate, TE; Kaper, JB; Boedeker, EC;
Indirizzi:
Univ Maryland, Sch Med, Ctr Vaccine Dev, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Vaccine Dev, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 l & Immunol, Baltimore, MD 21201 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 4, volume: 69, anno: 2001,
pagine: 2107 - 2115
SICI:
0019-9567(200104)69:4<2107:CNSAAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATTACHING-EFFACING LESIONS; COMPLETE DNA-SEQUENCE; PATHOGENICITY ISLAND; EPITHELIAL-CELLS; GENETIC-LOCUS; CONTROLS EXPRESSION; III SECRETION; HEP-2 CELLS; HOST-CELLS; INTIMIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Boedeker, EC Univ Maryland, Sch Med, Ctr Vaccine Dev, 685 W Baltimore St, Baltimore, MD21201 USA Univ Maryland 685 W Baltimore St Baltimore MD USA 21201 1 USA
Citazione:
C.R. Zhu et al., "Complete nucleotide sequence and analysis of the locus of enterocyte effacement from rabbit diarrheagenic Escherichia coli RDEC-1", INFEC IMMUN, 69(4), 2001, pp. 2107-2115

Abstract

The pathogenicity island termed the locus of enterocyte effacement (LEE) is found in diverse attaching and effacing pathogens associated with diarrhea in humans and other animal species. To explore the relation of variation in LEE sequences to host specificity and genetic lineage, we determined thenucleotide sequence of the LEE region from a rabbit diarrheagenic Escherichia coli strain RDEC-1 (O15:H-) and compared it with those from human enteropathogenic E. coli (EPEC, O127:H6) and enterohemorrhagic E. coli (EHEC, O157:H7) strains. Differing from EPEC and EHEC LEEs, the RDEC-1 LEE is not inserted at selC and is flanked by an IS2 element and the lifA toxin gene. The RDEC-1 LEE contains a core region of 40 open reading frames, all of whichare shared with the LEE of EPEC and EHEC. orf3 and the ERIC (enteric repetitive intergenic consensus) sequence present in the LEEs of EHEC and EPEC are absent from the RDEC-1 LEE. The predicted promoters of LEE1, LEE2, LEE3,tir, and LEE4 operons are highly conserved among the LEEs, although the upstream regions varied considerably for tir and the crucial LEE2 promoter, suggesting differences in regulation. Among the shared genes, high homology (>95% identity) between the RDEC-1 and the EPEC and EHEC LEEs at the predicted amino acid level was observed for the components of the type LII secretion apparatus, the Ces chaperones, and the Ler regulator. In contrast, moredivergence (66 to 88% identity) was observed in genes encoding proteins involved in host interaction, such as intimin (Eae) and the secreted proteins(Tir and Esps). A comparison of the highly variable genes from RDEC-1 withthose from a number of attaching and effacing pathogens infecting different species and of different evolutionary lineages was performed. Although RDEC-1 diverges from some human-infecting EPEC and EHEC, most of the variation observed appeared to be due to evolutionary lineage rather than host specificity. Therefore, much of the observed hypervariability in genes involvedin pathogenesis may not represent specific adaptation to different host species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:29:11