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Titolo:
Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture
Autore:
Yang, SH; Tamai, R; Akashi, S; Takeuchi, O; Akira, S; Sugawara, S; Takada, H;
Indirizzi:
Tohoku Univ, Sch Dent, Dept Microbiol & Immunol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan Saga Med Sch, Dept Immunol, Saga 8498501, Japan Saga Med Sch Saga Japan 8498501 d Sch, Dept Immunol, Saga 8498501, Japan Osaka Univ, Microbial Dis Res Inst, Dept Host Def, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ost Def, Suita, Osaka 5650871, Japan
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 4, volume: 69, anno: 2001,
pagine: 2045 - 2053
SICI:
0019-9567(200104)69:4<2045:SEOMWL>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOLL-LIKE RECEPTOR-2; ENTEROCOCCUS-HIRAE ATCC-9790; HUMAN GINGIVAL FIBROBLASTS; MURAMYL-DIPEPTIDE; CUTTING EDGE; ANAPHYLACTOID REACTIONS; STRUCTURAL REQUIREMENTS; PERITONEAL-MACROPHAGES; INNATE IMMUNITY; WALL COMPONENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Takada, H Tohoku Univ, Sch Dent, Dept Microbiol & Immunol, Aoba Ku, 4-1 Seiryo Machi, Sendai, Miyagi 9808575, Japan Tohoku Univ 4-1 Seiryo Machi Sendai Miyagi Japan 9808575 , Japan
Citazione:
S.H. Yang et al., "Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture", INFEC IMMUN, 69(4), 2001, pp. 2045-2053

Abstract

An analog of 1 alpha ,25-dihydroxyvitamin D-3, 22-oxyacalcitriol (OCT), differentiated human monocytic THP-1 and U937 cells to express membrane CD14 and rendered the cells responsive to bacterial cell surface components, Both THP-1 and U937 cells expressed Toll-like receptor 4 (TLR4) on the cell surface and TLR4 mRNA in the cells, irrespective of OCT treatment. In contrast, OCT-treated U937 cells scarcely expressed TLR2 mRNA, while OCT-treated THP-1 cells expressed this transcript. Muramyldipeptide (MDP) by itself exhibited only a weak ability to induce secretion of inflammatory cytokines such as interleukin-8 (IL-8) in the OCT-differentiated THP-1 cells but showed marked synergistic effects with Salmonella lipopolysaccharide (LPS) or lipoteichoic acid (LTA) from Staphylococcus aureus, both of which exhibited strong activities. Combinatory stimulation with LPS plus LTA did not show a synergistic effect on OCT-differentiated THP-1 cells. Similar results were observed in OCT-differentiated U937 cells, although combination experiments were carried out only with MDP plus LPS. Anti CD14 monoclonal antibody (MAb)MY4, anti-TLR4 MAb HTA125, and the synthetic lipid A precursor LA-14-PP almost completely inhibited the IL-8-inducing activities of LTA as well as LPS on OCT-treated THP-1 cells, but these treatments increased MDP activity. OCT-treated THP-1 cells primed,vith MDP exhibited enhanced production of IL-8 upon stimulation with LPS, while the cells primed,vith LPS showed no change in production upon stimulation with MDP. MDP up-regulated mRNA expression of an adapter molecule to TLRs, MyD88, to an extent similar to that for LPS in OCT-treated THP-1 cells. These findings suggested that LTA as well as LPS activated human monocytic tells in a CD14- and TLR4-dependent manner,whereas MDP exhibited activity in a CD14-, TLR4-, and probably TLR2-independent manner and exhibited synergistic and priming effects on the cells forcytokine production in response to various bacterial components.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 14:50:26