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Titolo:
Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A
Autore:
Chung, JY; Wilkie, I; Boyce, JD; Townsend, KM; Frost, AJ; Ghoddusi, M; Adler, B;
Indirizzi:
Monash Univ, Dept Microbiol, Bacterial Pathogenesis Res Grp, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 Grp, Clayton, Vic 3800, Australia Univ Queensland, Sch Vet Sci & Anim Prod, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 4, volume: 69, anno: 2001,
pagine: 2487 - 2492
SICI:
0019-9567(200104)69:4<2487:ROCITP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
B NEISSERIA-MENINGITIDIS; SERUM RESISTANCE; ESCHERICHIA-COLI; HYALURONIC-ACID; BACTERICIDAL ACTIVITY; VIRULENCE; PHAGOCYTOSIS; ENCAPSULATION; STREPTOCOCCUS; NEUTROPHILS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Adler, B Monash Univ, Dept Microbiol, Bacterial Pathogenesis Res Grp, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 yton, Vic 3800, Australia
Citazione:
J.Y. Chung et al., "Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A", INFEC IMMUN, 69(4), 2001, pp. 2487-2492

Abstract

We have constructed a defined acapsular mutant in Pasteurella multocida X-73 (serogroup A:1) by disrupting the hexA gene through the insertion of a tetracycline resistance cassette, The genotype of the hexA::tet(M) strain was confirmed by PCR and Southern hybridization, and the acapsular phenotype of this strain was confirmed by electron microscopy, The hexA::tet(M) strain was attenuated in both mice and chickens. Complementation of the mutant with an intact hexAB fragment restored lethality in mice but not in chickens. In contrast to the results described previously for P. multocida serogroup B (J. D. Boyce and B. Adler, Infect. Immun, 68:3463-3468, 2000), the hexA::tet(M) strain was sensitive to the bactericidal action of chicken serum, whereas the wildtype and complemented strains were both resistant. Following inoculation into chicken muscle, the bacterial count of the hexA:tet(M) strain decreased significantly, while the wild-type and complemented strainsboth grew rapidly over 4 h, The capsule is thus an essential virulence determinant in the pathogenesis of fowl cholera.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:41:55