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Titolo:
Measurement of 8-oxo-2'-deoxyguanosine and 8-oxo-2'-deoxyadenosine in DNA and human urine by high performance liquid chromatography-electrospray tandem mass spectrometry
Autore:
Weimann, A; Belling, D; Poulsen, HE;
Indirizzi:
Rigshosp, Dept Clin Pharmacol, DK-2100 Copenhagen O, Denmark Rigshosp Copenhagen Denmark O n Pharmacol, DK-2100 Copenhagen O, Denmark
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 7, volume: 30, anno: 2001,
pagine: 757 - 764
SICI:
0891-5849(20010401)30:7<757:MO8A8I>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HPLC-ELECTROCHEMICAL DETECTION; FAST-ATOM-BOMBARDMENT; BASE DAMAGE; CHEMICAL DETERMINATION; QUANTITATIVE-ANALYSIS; CELLULAR DNA; HUMAN-CELLS; 8-HYDROXY-2'-DEOXYGUANOSINE; 8-OXOGUANINE; ADDUCTS;
Keywords:
8-oxo-2 '-deoxyguanosine; 8-oxo-2 '-deoxyadenosine; oxidative stress; nucleosides; electrospray; isotope dilution; free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Poulsen, HE Rigshosp, Dept Clin Pharmacol, Q7642,Blegdamsvej 9, DK-2100 Copenhagen O, Denmark Rigshosp Q7642,Blegdamsvej 9 Copenhagen Denmark O O, Denmark
Citazione:
A. Weimann et al., "Measurement of 8-oxo-2'-deoxyguanosine and 8-oxo-2'-deoxyadenosine in DNA and human urine by high performance liquid chromatography-electrospray tandem mass spectrometry", FREE RAD B, 30(7), 2001, pp. 757-764

Abstract

A method for the determination of 8-oxo-2'-deoxyguanosine and 8-oxo-2'-deoxyadenosine in DNA and urine by High Performance Liquid Chromatography (HPLC)-Tandem Mass Spectrometry is described. For the urine samples there is nosample preparation except for addition of buffer and internal standards followed by redissolvation of precipitate containing 8-oxo-2'-deoxyguanosine and a centrifugation step before the samples are injected onto the HPLC column. The detection Limit for 8-oxo-2'-deoxyguanosine and 8-oxo-2'-deoxyadenosine is approximately 0.3 nM corresponding to 7.5 fmol injected. Long runs, that is, > 50 samples, can be analyzed with only minimal loss of sensitivity. The concentrations excreted into urine samples from humans are between1 and 100 nM for 8-oxo-2'-deoxyguanosine and below 0.3 nM for 8-oxo-2'-deoxyadenosine. In calf thymus DNA levels down to about 1 oxidized guanosine and adenosine per 10(6) unmodified bases can be detected. High levels of 8-oxo-2'-deoxyguanosine were found, 30 per 10(6) 2'-deoxyguanosine, levels of 8-oxo-2'-deoxyadenosine are at or below the detection limit. These findingsindicate that High Performance Liquid Chromatography-Tandem Mass Spectrometry is a highly sensitive and specific method for analysis of oxidative DNAmodifications in tissue as well as for analysis of excretion of oxidized nucleotides into urine that ensures a minimum artifact formation. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 11:29:43