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Titolo:
The stability of cytokeratin 18 in human liver cells during colchicine-induced microtubule disruption
Autore:
Liu, YH; Su, B; Pei, RJ; Yeh, CC; Yeh, KT; Lee, KY; Hsu, YH; Ho, CC; Lai, YS;
Indirizzi:
China Med Coll, Sch Med, Dept Pathol, Taichung, Taiwan China Med Coll Taichung Taiwan , Sch Med, Dept Pathol, Taichung, Taiwan China Med Coll Hosp, Dept Urol, Taichung, Taiwan China Med Coll Hosp Taichung Taiwan l Hosp, Dept Urol, Taichung, Taiwan Changha Christian Hosp, Dept Pathol, Changhua, Taiwan Changha Christian Hosp Changhua Taiwan p, Dept Pathol, Changhua, Taiwan Jen Ai Hosp, Dept Pathol, Taichung, Taiwan Jen Ai Hosp Taichung TaiwanJen Ai Hosp, Dept Pathol, Taichung, Taiwan Tzu Chi Hosp, Dept Pathol, Hua Lien, Taiwan Tzu Chi Hosp Hua Lien Taiwan zu Chi Hosp, Dept Pathol, Hua Lien, Taiwan China Med Coll, Sch Med, Dept Physiol, Taichung, Taiwan China Med Coll Taichung Taiwan Sch Med, Dept Physiol, Taichung, Taiwan
Titolo Testata:
FOOD AND CHEMICAL TOXICOLOGY
fascicolo: 1, volume: 39, anno: 2001,
pagine: 85 - 89
SICI:
0278-6915(200101)39:1<85:TSOC1I>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENTS; TUMOR TRANSFORMATION; EXPRESSION; ACTIN; EPITHELIA; PROTEINS; MOLECULE; HEPATOMA;
Keywords:
cytokeratin; modulation; hepatoma; microtubule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Lai, YS China Med Coll, Sch Med, Dept Pathol, 91 Hsueh Shih Rd, Taichung, Taiwan China Med Coll 91 Hsueh Shih Rd Taichung Taiwan Taichung, Taiwan
Citazione:
Y.H. Liu et al., "The stability of cytokeratin 18 in human liver cells during colchicine-induced microtubule disruption", FOOD CHEM T, 39(1), 2001, pp. 85-89

Abstract

The cytoskeleton plays important roles in eel function and is therefore implicated in the pathogenesis of many human liver diseases, including malignant tumors. The stability of cytokeratin proteins during tumor transformation in human hepatocellular carcinoma has been studied with a molecular approach previously. The results demonstrate that the cytokeratin is modulated in human hepatocellular carcinoma. Besides this, three low molecular weightcytokeratin molecules (named HCC CK) are found. This indicates that these HCC CKs have undergone modulation from the human hepatocyte cytokeratin Is. We also checked the cytokeratin profile of the human hepatoma cell line PLC/PRF/5 with the same methods to ensure the HCC CK molecules are produced by modulation but not protein degradation. The stability of cytokeratin molecules was studied by a different approach. The cytokeratin compositions of human liver cells (Chang cell line) were analysed under the effects of microtubule-disrupting drug (colchicine) by SDS-PAGE, Western blot, immunoprecipitation using a commercially available monoclonal anti-cytokeratin 18 antibody and immunofluorescent staining, Within 1 h of treatment, the microtubule began to collapse and the filamentous structure was shortening. The microtubule had almost collapsed and became fragmented to form a lattice-like network after 24 h of treatment. The cytokeratin was modulated after long-term (24 h) treatment of colchicine, and the molecular weight became 14 kD and the antigenicity was lost. The stability of cytokeratin molecules was related to the intact microtubule network, after disruption of the microtubulethe cytokeratin would be modulated. The intact microtubule network was a stabilizing factor of cytokeratin 18 in human liver cells. (C) 2001 ElsevierScience Ltd, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:22:59