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Titolo:
RELATIONSHIP BETWEEN CARDIAC-FUNCTION AND SUBSTRATE OXIDATION IN HEARTS OF DIABETIC RATS
Autore:
CHATHAM JC; FORDER JR;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT RADIOL,NMR RES DIV,217 TRAYLOR BLDG,720 RUTLAND AVE BALTIMORE MD 21205
Titolo Testata:
American journal of physiology. Heart and circulatory physiology
fascicolo: 1, volume: 42, anno: 1997,
pagine: 52 - 58
SICI:
0363-6135(1997)42:1<52:RBCASO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-13 NMR-SPECTROSCOPY; GLUCOSE-OXIDATION; PYRUVATE-DEHYDROGENASE; PERFUSED HEART; ACID CYCLE; CARDIOMYOPATHY; METABOLISM; DICHLOROACETATE; GLYCOLYSIS; MELLITUS;
Keywords:
HEXANOATE; PYRUVATE DEHYDROGENASE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Chatham e J.R. Forder, "RELATIONSHIP BETWEEN CARDIAC-FUNCTION AND SUBSTRATE OXIDATION IN HEARTS OF DIABETIC RATS", American journal of physiology. Heart and circulatory physiology, 42(1), 1997, pp. 52-58

Abstract

The effects of streptozotocin-induced diabetes on myocardial substrate oxidation and contractile function were investigated using C-13 nuclear magnetic resonance (NMR) spectroscopy. To determine the consequences of diabetes on glucose oxidation, hearts were perfused with [1-C-13]glucose (11 mM) alone as well as in the presence of insulin ito stimulate glucose transport) and dichloroacetate itc, stimulate pyruvate dehydrogenase). The contribution of glucose to the tricarboxylic acid (TCA) cycle was significantly decreased in hear ts from diabetic animalscompared with controls, with glucose alone and with insulin; however,the addition of dichloroacetate significantly increased the contribution of glucose to the TCA cycle. Contractile function in hearts from diabetic animals was significantly depressed with glucose as the sole substrate, regardless of the presence of insulin or dichloroacetate (P < 0.0005). To determine whether diabetes had any direct effects on beta-oxidation and the TCA cycle, hearts were perfused with glucose (11 mM) plus [6-C-13]hexanoate (0.5 mM) as substrates. In control hearts, with glucose plus hexanoate as substrates, hexanoate contributed 98.9 +/- 2% of the substrate entering the TCA cycle; this was significantly decreased to 90.7 +/- 0.6% in the diabetic group (P < 0.02). The addition of hexanoate to the perfusate resulted in a significant increase in peak systolic pressure in the diabetic group (P < 0.001) such that contractile function was indistinguishable from controls.

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Documento generato il 25/11/20 alle ore 04:32:52