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Titolo:
Subfield-specific immediate early gene expression associated with hippocampal long-term potentiation in vivo
Autore:
French, PJ; OConnor, V; Jones, MW; Davis, S; Errington, ML; Voss, K; Truchet, B; Wotjak, C; Stean, T; Doyere, V; Maroun, M; Laroche, S; Bliss, TVP;
Indirizzi:
Natl Inst Med Res, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA ed Res, London NW7 1AA, England Univ Paris Sud, CNRS, UMR 8620, F-91405 Orsay, France Univ Paris Sud Orsay France F-91405 NRS, UMR 8620, F-91405 Orsay, France SmithKline Beecham Pharmaceut, Harlow CM19 5AW, Essex, England SmithKline Beecham Pharmaceut Harlow Essex England CM19 5AW ssex, England
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 5, volume: 13, anno: 2001,
pagine: 968 - 976
SICI:
0953-816X(200103)13:5<968:SIEGEA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENTATE GYRUS; SYNAPTIC ACTIVITY; GROWTH-FACTOR; LATE-PHASE; PROTEIN-SYNTHESIS; RAT HIPPOCAMPUS; MESSENGER-RNA; INDUCTION; MAINTENANCE; BRAIN;
Keywords:
CA1; dentate gyrus; immediate early gene; long-term potentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: French, PJ Erasmus Univ, Dept Anat, POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ POB 1738 Rotterdam Netherlands NL-3000 DR erlands
Citazione:
P.J. French et al., "Subfield-specific immediate early gene expression associated with hippocampal long-term potentiation in vivo", EUR J NEURO, 13(5), 2001, pp. 968-976

Abstract

It is not known whether NMDA receptor-dependent long-term potentiation (LTP) is mediated by similar molecular mechanisms in different hippocampal areas. To address this question we have investigated changes in immediate early gene and protein expression in two hippocampal subfields following the induction of LTP in vivo and in vitro. In granule cells of the dentate gyrus,LTP induced in vivo by tetanic stimulation of the perforant path was followed by strong induction of the immediate early genes (IEGs) Zif268, Arc andHomer. The increase in Zif268 mRNA was accompanied by an increase in protein expression. In contrast, we were unable to detect modulation of the IEGsZif268, Arc, Homer and HB-GAM following induction of LTP by high-frequencystimulation of the commissural projection to CA1 pyramidal cells in vivo. In this pathway, we also failed to detect modulation of Zif268 protein levels. Zif268, Arc and Homer can be modulated in CA1 pyramidal cells approximately twofold after electroshock-induced maximal seizure, which demonstratespotential responsiveness to electrical stimuli. When LTP was induced in vitro neither CA1 pyramidal cells nor granule cells showed an increase in Zif268, Arc or Homer mRNA. However, in the slice preparation, granule cells have a different transcriptional state as basal IEG levels are elevated. These results establish the existence of subfield-specific transcriptional responses to LTP-inducing stimulation in the hippocampus of the intact animal, and demonstrate that in area CA1-enhanced transcription of Zif268, Arc and Homer is not required for the induction of late LTP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:31:53