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Titolo:
Overexpression of the myristoylated alanine-rich C kinase substrate decreases uptake and K+-evoked release of noradrenaline in the human neuroblastoma SH-SY5Y
Autore:
Hartness, ME; Wade, JA; Walker, JH; Vaughan, PFT;
Indirizzi:
Univ Leeds, Inst Cardiovasc Res, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 5, volume: 13, anno: 2001,
pagine: 925 - 934
SICI:
0953-816X(200103)13:5<925:OOTMAC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSE-CORED VESICLES; H-3 NORADRENALINE; ANGIOTENSIN-II; MARCKS FAMILY; CELL-LINES; ACTIVATION; ALPHA; CALMODULIN; CARBACHOL; INCREASES;
Keywords:
exocytosis; protein kinase C substrate; stable transfected cell line; sympathetic neuron;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Vaughan, PFT Univ Leeds, Inst Cardiovasc Res, Worsley Med & Dent Bldg, Leeds LS2 9JT, WYorkshire, England Univ Leeds Worsley Med & Dent Bldg Leeds W Yorkshire England LS2 9JT
Citazione:
M.E. Hartness et al., "Overexpression of the myristoylated alanine-rich C kinase substrate decreases uptake and K+-evoked release of noradrenaline in the human neuroblastoma SH-SY5Y", EUR J NEURO, 13(5), 2001, pp. 925-934

Abstract

The aim of this study was to investigate a possible role of the myristoylated alanine-rich C kinase substrate (MARCKS) in the mechanism of noradrenaline uptake and release in the human neuroblastoma cell line SH-SY5Y. A stable cell line showing a twofold overexpression of MARCKS was prepared by transfecting SH-SY5Y with pCEP4 containing MARCKS cDNA in the sense orientation. This cell line showed no changes in the expression of neurofilaments or markers of noradrenergic large dense-cored vesicles compared with both untransfected SH-SY5Y and SH-SY5Y transfected with pCEP4 only (mock transfected). Similarly, no differences in the rate of cell growth could be detected between these three cell lines. In contrast, specific uptake and depolarization-evoked (100 mm K+) release of noradrenaline from the cell line overexpressing MARCKS was inhibited by approximately 50% compared with mock-transfected SH-SY5Y. K+-evoked noradrenaline release enhanced by pretreatment with12-O-tetradecanoylphorbol 13-acetate (100 nm) was also inhibited by 50%. In contrast, carbachol-evoked noradrenaline release was unaffected. Thus, inSH-SY5Y cells, overexpression of MARCKS leads to a decrease in the K+-evoked noradrenaline release possibly by increased actin cross-linking preventing the movement of noradrenaline containing large dense-cored vesicles to the plasma membrane in response to depolarization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:27:24